首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠翻译调节肿瘤蛋白(TPT1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的TPT1含量。TPT1是一个重要靶点。它与细胞内多种生理过程相关，在肿瘤发生、发展等方面可能发挥作用。研究机制主要聚焦于其在细胞增殖、凋亡调控通路中的作用，探索其作为肿瘤诊断标志物和治疗靶点的可能，为相关疾病治疗提供新思路。试剂盒检测范围为39 pg/mL-2500 pg/mL，适用于肿瘤生物学研究中的蛋白质表达分析、药物干预模型评价以及细胞应激状态下TPT1动态监测等科研场景，为探索肿瘤发生机制及筛选潜在治疗靶点提供精准的体外检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Tpt1 ELISA Kit; Trt ELISA Kit; Translationally-controlled tumor protein ELISA Kit; TCTP ELISA Kit; Lens epithelial protein ELISA Kit
缩写：TPT1
Uniprot No.：P63029
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：39 pg/mL-2500 pg/mL
灵敏度：9.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TPT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 94-104 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 88-99 | |||||
回收率：
| The recovery of rat TPT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 89-104 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected25002.0472.1342.0911.95912501.3841.4831.4341.3026250.8540.8360.8450.713312.50.5170.5240.5210.3891560.3370.3460.3420.210780.2570.2480.2530.121390.1950.2010.1980.06600.1340.1290.132 | ||||||
货期：3-5 working days
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