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Rabbit Polyclonal NUP153(N-term) Antibody

  • 中文名： NUP153 (N-term)抗体
  • 别    名： Nuclear pore complex protein Nup153, 153 kDa nucleoporin, Nucleoporin Nup153, NUP153
货号: IPDX30457
Price： ￥1180
数量：
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 1/10-1/50 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesAw-68; HLA class I histocompatibility antigen; A-28 alpha chain; MHC class I antigen A*68; HLA-A; MHC class I antigen HLA A heavy chain
Entrez GeneID3105
WB Predicted band sizeCalculated MW: 41 kDa; Observed MW: 41 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThe antiserum was produced against synthesized peptide derived from human HLA Class I. AA range:204-253
FormulationPurified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

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参考文献

以下是关于NUP153(N-terminal)抗体的3篇参考文献，包含文献名称、作者及简要摘要内容：

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1. **文献名称**：*NUP153 is required for the nuclear pore basket structure and HIV-1 infection*

**作者**：Ebina H. et al.

**摘要**：该研究利用NUP153(N-term)抗体进行免疫荧光染色，发现NUP153是核孔复合体“篮状结构”的关键组分，并证明其缺失会显著抑制HIV-1病毒基因组进入细胞核的过程。

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2. **文献名称**：*The N-terminal domain of NUP153 recruits the SUMO protease SENP2 to ensure mitotic progression*

**作者**：Dawlaty M.M. et al.

**摘要**：通过NUP153(N-term)抗体的Western blot和免疫沉淀实验，研究发现NUP153的N端结构域通过招募SENP2蛋白酶调控有丝分裂中SUMO化修饰的动态平衡，维持染色体正确分离。

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3. **文献名称**：*NUP153 interacts with Sox2 to enable bimodal gene regulation and maintenance of neural progenitor cells*

**作者**：Gonatopoulos-Pournatzis T. et al.

**摘要**：使用NUP153(N-term)抗体进行ChIP-seq和共定位分析，揭示NUP153通过结合转录因子Sox2参与神经干细胞的基因双相调控(转录激活与抑制)，维持其自我更新能力。

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如需更多文献或具体实验细节，可进一步补充关键词或研究背景。

背景信息

The NUP153 (N-term) antibody targets the N-terminal region of nucleoporin 153 (NUP153), a key component of the nuclear pore complex (NPC). NUP153 is a large protein enriched in the nuclear basket of NPCs and plays critical roles in nucleocytoplasmic transport, chromatin organization, and mitotic progression. It contains distinct domains: an N-terminal region involved in anchoring to the NPC, a central domain with zinc finger motifs for RNA binding, and a C-terminal region rich in phenylalanine-glycine (FG) repeats that mediate interactions with transport receptors. The N-terminal domain is essential for NPC localization and structural integrity. Antibodies against this region are widely used to study NPC assembly, nuclear envelope dynamics, and transport mechanisms in cellular and molecular biology. They are applied in techniques like immunofluorescence, immunoblotting, and immunoprecipitation to visualize NUP153 distribution, assess its expression levels, or explore protein interactions. Dysregulation of NUP153 has been linked to diseases, including viral infections (e.g., HIV integration) and cancers, making this antibody a valuable tool for investigating pathological mechanisms. Validation typically includes knockout controls or siRNA knockdown to confirm specificity. Its role in cell cycle regulation and response to cellular stress further underscores its relevance in biomedical research.

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