Recombinant Human YWHAB protein

YWHAB, a member of the 14-3-3 protein family, is a highly conserved regulatory protein involved in diverse cellular processes, including signal transduction, cell cycle control, apoptosis, and metabolism. The 14-3-3 family consists of seven isoforms (β, γ, ε, η, σ, τ/θ, ζ) encoded by distinct genes. YWHAB, also designated as 14-3-3β, functions as a phosphoserine/phosphothreonine-binding protein that interacts with numerous client proteins to modulate their activity, localization, or stability. It acts as a molecular scaffold or chaperone, facilitating protein-protein interactions critical for cellular homeostasis. Dysregulation of YWHAB has been implicated in cancer, neurodegenerative diseases, and metabolic disorders, underscoring its biological significance.

Recombinant YWHAB protein is engineered through heterologous expression systems, such as *E. coli* or mammalian cell cultures, to produce purified, functional protein for research and therapeutic applications. The recombinant form typically retains the native structure, enabling studies on its binding partners, post-translational modifications, and mechanistic roles in signaling pathways. Its production often involves affinity chromatography (e.g., GST or His-tag systems) followed by rigorous quality control to ensure purity and bioactivity.

In research, recombinant YWHAB is widely used to investigate its interactions with kinases (e.g., RAF, PI3K), tumor suppressors (e.g., p53), and apoptosis regulators (e.g., BAD, BAX). It also serves as a tool to explore its role in diseases; for example, its overexpression in cancers correlates with chemoresistance, while reduced levels are linked to neurodegeneration. Additionally, recombinant YWHAB supports drug discovery efforts targeting 14-3-3 protein complexes. Its versatility makes it indispensable for dissecting cellular pathways and developing diagnostic or therapeutic strategies.