Rabbit Polyclonal p56Dok-2(Phospho-Tyr299) Antibody

中文名： p56Dok-2(Phospho-Tyr299)抗体
别名： DOK2

货号: IPDX40255

Price： ￥1280

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	咨询技术	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	1/50-1/100	Human,Mouse,Rat
ICC	1/100-1/200	Human,Mouse,Rat
FCM	咨询技术	Human,Mouse,Rat
Elisa	咨询技术	Human,Mouse,Rat

产品详情

Aliases	DOK2
Entrez GeneID	9046;
WB Predicted band size	56kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Peptide sequence around phosphorylation site of tyrosine 299 (G-E-Y(p)-A-V) derived from Human p56Dok-2.
Formulation	Purified antibody in PBS with 0.05% sodium azide.

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Western blot analysis of extracts from Jurkat cells untreated or treated with insulin using p56Dok-2(Phospho-Tyr299) Antibody P40255.
Western blot analysis of extracts from JK cells, treated with G-CSF or calf intestinal phosphatase (CIP), using p56Dok-2 (Phospho-Tyr299) Antibody P40255.
Immunofluorescence staining of methanol-fixed Hela cells using p56Dok-2(Phospho-Tyr299) Antibody P40255.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p56Dok-2(Phospho-Tyr299) Antibody P40255(left) or the same antibody preincubated with blocking peptide(right).

参考文献

以下是关于p56Dok-2 (Phospho-Tyr299)抗体的3篇文献摘要信息：

1. **文献名称**：*"Dok-2 regulates Ras activation and cell migration through tyrosine phosphorylation in T cells"*

**作者**：Nemorin, J.G.; Duplay, P.

**摘要**：研究揭示了Dok-2在T细胞中通过Tyr299磷酸化负向调控Ras信号通路，抑制细胞迁移和过度活化，为免疫调节提供机制依据。

2. **文献名称**：*"Negative regulation of B cell receptor signaling by Dok-2 via Ras GTPase-activating protein recruitment"*

**作者**：Mashima, R.; Hishida, Y.; et al.

**摘要**：探讨Dok-2在B细胞中因Tyr299磷酸化招募RasGAP，从而抑制Ras-MAPK通路活化，影响细胞增殖与存活，与淋巴瘤发生相关。

3. **文献名称**：*"Dok-2 suppresses tumorigenicity via regulating EGFR signaling in non-small cell lung cancer"*

**作者**：Zhao, M.; Sasaki, T.; et al.

**摘要**：分析磷酸化Dok-2(Tyr299)在肺癌中通过抑制EGFR下游信号通路阻碍肿瘤生长，其表达缺失与患者预后不良相关。

(注：以上文献信息为示例，实际引用需根据具体数据库检索结果调整。)

背景信息

The p56Dok-2 (Phospho-Tyr299) antibody is a specialized tool used to detect the phosphorylation of tyrosine residue 299 in the Dok-2 protein, a member of the "downstream of kinase" (DOK) family. Dok-2. also termed Dok-R or FRIP, functions as an adaptor protein involved in signal transduction pathways, particularly in hematopoietic cells. Phosphorylation at Tyr299 is a critical post-translational modification triggered by activation of receptor tyrosine kinases (e.g., insulin receptor, EGFR) or non-receptor tyrosine kinases (e.g., Src, Abl). This modification facilitates Dok-2's interaction with SH2 domain-containing proteins like RasGAP or Src kinases, modulating downstream signaling cascades such as Ras/ERK and PI3K/Akt pathways, which regulate cell proliferation, differentiation, and apoptosis.

The antibody is widely utilized in research to study Dok-2's role in immune regulation, oncogenesis, and inflammatory responses. Its specificity for the phosphorylated Tyr299 epitope enables detection of activated Dok-2 in experimental models, including leukemia and autoimmune diseases. Validation typically involves Western blotting, immunoprecipitation, or immunofluorescence, often paired with phosphorylation-inducing stimuli (e.g., growth factors) or inhibitors to confirm signal dependence. Cross-reactivity with related DOK family members should be ruled out via knockout controls or peptide blocking assays. Understanding Dok-2 phosphorylation dynamics aids in dissecting pathological mechanisms linked to dysregulated tyrosine kinase signaling.