| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CD122 antigen; P70-75; IL-2 receptor; IL-2R beta; |
| Entrez GeneID | 3560; |
| WB Predicted band size | 75kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 364(Q-G-Y(p)-F-F) derived from Human IL-2Rβ/CD122 . |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于IL-2Rβ/CD122 (Phospho-Tyr364)抗体的3篇参考文献及其摘要内容的简要整理：
1. **"IL-2 Receptor β Chain Tyrosine Phosphorylation Regulates T Cell Proliferation"**
*作者：Smith A, et al.*
摘要：该研究揭示了IL-2Rβ链Tyr364位点的磷酸化在T细胞活化中的关键作用。通过使用特异性抗Phospho-Tyr364抗体进行免疫印迹分析，发现该位点的磷酸化是IL-2信号转导及下游STAT5激活的必要条件，阻断此磷酸化显著抑制T细胞增殖。
2. **"Phosphorylation Dynamics of CD122 in NK Cell Activation"**
*作者：Wang L, et al.*
摘要：研究利用Phospho-Tyr364抗体探究自然杀伤(NK)细胞中CD122的磷酸化动态。结果表明，Tyr364磷酸化水平与NK细胞的细胞毒性功能直接相关，抗体阻断实验证实该位点通过调控PI3K/Akt通路影响免疫应答。
3. **"Structural Basis of IL-2 Receptor β Chain Signaling via Tyr364 Phosphorylation"**
*作者：Tanaka K, et al.*
摘要：通过晶体学分析和基于Phospho-Tyr364抗体的功能实验，本研究阐明了IL-2Rβ链Tyr364磷酸化后的构象变化，揭示了其招募衔接蛋白Shc并激活MAPK通路的分子机制，为靶向该位点的免疫治疗提供理论依据。
(注：以上文献为示例性内容，实际引用需根据具体研究补充真实来源。)
The IL-2Rβ/CD122 (Phospho-Tyr364) antibody is a specialized tool used to study the activation and signaling of the interleukin-2 receptor beta subunit (IL-2Rβ/CD122), a critical component of the high-affinity IL-2 receptor complex. IL-2Rβ, encoded by the *IL2RB* gene, pairs with the common gamma chain (γc, CD132) to mediate IL-2 signaling in immune cells, particularly T cells and NK cells. Phosphorylation of tyrosine 364 (Tyr364) in the cytoplasmic domain of IL-2Rβ is a key regulatory event triggered by IL-2 binding. This post-translational modification facilitates downstream signaling through JAK-STAT, MAPK, and PI3K-Akt pathways, driving T-cell proliferation, survival, and differentiation.
The antibody specifically recognizes the phosphorylated form of Tyr364. enabling researchers to detect and quantify IL-2Rβ activation in experimental models. It is widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate IL-2-mediated immune responses, dysregulated signaling in autoimmune disorders (e.g., multiple sclerosis), or cancer immunotherapy mechanisms. Its specificity for the phosphorylated epitope ensures minimal cross-reactivity with non-activated IL-2Rβ, making it a reliable biomarker for studying receptor dynamics. Applications extend to drug development, where it helps assess compounds targeting IL-2 pathways for modulating immune cell activity.
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