Rabbit Polyclonal HDAC3(Phospho-Ser424) Antibody

The HDAC3 (Phospho-Ser424) antibody is a specialized tool used to study the post-translational modification of histone deacetylase 3 (HDAC3), a critical enzyme in epigenetic regulation. HDAC3. a member of the class I HDAC family, catalyzes the removal of acetyl groups from histones and non-histone proteins, modulating gene expression, chromatin structure, and diverse cellular processes such as proliferation, differentiation, and apoptosis. Phosphorylation at serine 424 (Ser424) is a key regulatory mechanism influencing HDAC3 activity, subcellular localization, or interactions with co-repressor complexes like NCoR/SMRT. This modification may alter HDAC3’s enzymatic function or its association with nuclear receptors, thereby impacting transcriptional repression. The HDAC3 (Phospho-Ser424) antibody specifically recognizes this phosphorylated epitope, enabling researchers to investigate the activation status or regulatory pathways of HDAC3 in physiological and pathological contexts. It is widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to assess phosphorylation dynamics in response to cellular signals (e.g., kinase activation) or therapeutic agents. Studies employing this antibody have shed light on HDAC3’s roles in cancer progression, metabolic disorders, and neurological diseases, where dysregulated phosphorylation may contribute to disease mechanisms. Proper validation, including knockout controls and peptide competition assays, is essential to ensure specificity due to potential cross-reactivity with related phosphorylated proteins.