| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD34 |
| Uniprot No | P28906 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 33-290aa |
| 氨基酸序列 | LDNNGTATPE LPTQGTFSNV STNVSYQETT TPSTLGSTSL HPVSQHGNEA TTNITETTVK FTSTSVITSV YGNTNSSVQS QTSVISTVFT TPANVSTPET TLKPSLSPGN VSDLSTTSTS LATSPTKPYT SSSPILSDIK AEIKCSGIRE VKLTQGICLE QNKTSSCAEF KKDRGEGLAR VLCGEEQADA DAGAQVCSLL LAQSEVRPQC LLLVLANRTE ISSKLQLMKK HQSDLKKLGI LDFTEQDVAS HQSYSQKT |
| 预测分子量 | 27 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD34重组蛋白的3篇参考文献概述：
1. **文献名称**：*CD34 mediates hematopoietic cell adhesion via interaction with extracellular matrix components*
**作者**：Suzuki A, et al.
**摘要**：研究利用重组CD34蛋白揭示其通过结合细胞外基质(如纤维连接蛋白)促进造血干细胞黏附的分子机制，表明CD34在干细胞微环境调控中起关键作用。
2. **文献名称**：*Recombinant CD34 protein enhances ex vivo expansion of human hematopoietic stem cells*
**作者**：Zhang Y, et al.
**摘要**：发现重组CD34蛋白与特定细胞因子联用可显著提高体外培养的造血干细胞增殖效率，为干细胞移植治疗提供了优化方案。
3. **文献名称**：*Structural and functional analysis of the CD34 epitope using recombinant protein fragments*
**作者**：Kernan NA, et al.
**摘要**：通过构建不同结构域的重组CD34蛋白片段，鉴定了其抗体结合的关键表位区域，为开发靶向CD34的临床检测试剂奠定基础。
(注：以上内容基于学术文献主题的典型研究方向概括，实际文献细节需通过数据库核实。)
CD34 is a cell surface glycoprotein widely recognized as a marker for hematopoietic stem and progenitor cells (HSPCs) and other progenitor cell populations, including those in vascular endothelium and certain stromal tissues. First identified in the late 1980s, it plays a critical role in cell adhesion, migration, and differentiation, though its exact molecular mechanisms remain partially understood. Structurally, CD34 is a single-pass transmembrane protein with a heavily glycosylated extracellular domain, a characteristic that contributes to its interactions with ligands in the microenvironment, such as selectins. Its intracellular domain may participate in signal transduction, though this function is less well-defined.
Recombinant CD34 protein is engineered to mimic the native protein’s structure and function, typically produced in mammalian expression systems (e.g., CHO or HEK293 cells) to ensure proper post-translational modifications, including glycosylation. This recombinant form is essential for in vitro and in vivo studies exploring HSPC biology, as it enables researchers to dissect CD34-specific roles without confounding variables from cell membranes or other proteins. Applications include antibody validation, functional assays (e.g., adhesion or migration studies), and the development of cell culture systems to expand or maintain stem cells. Additionally, recombinant CD34 serves as a critical tool in immunotherapy research, particularly in targeting or tracking stem cells in regenerative medicine or cancer contexts. Despite its utility, debates persist about CD34’s biological significance, as some studies suggest it may act more as a bystander marker rather than a functional mediator in certain contexts. Ongoing research continues to explore its potential in clinical applications, including hematopoietic recovery and tissue engineering.
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