| 纯度 | >95%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | CNTNAP2 |
| Uniprot No | Q9CPW0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1262aa |
| 氨基酸序列 | MVMSLRAGYR AALSLWILSS FICRAWTAPS TFQKCDEPLI SGLPHVSFSS SSSLSSSYAP GYAKINKRGG AGGWSPSDSD HYQWLQVDFG NRKQISAIAT QGRYSSSDWV TQYRMLYSDT GRNWKPYHQD GNIWAFPGNI NSDSVVRHDL QHAVVARYVR IVPLDWNGEG HIGLRAEVYG CAYWADVINF DGHGVLPYRF RNKKMKTLKD VIALKFKTSE SEGVLLHGEG QQGDYITLEL KKAKLVLSLN LGSNQLGPIY GHTSVTSGSL LDDHHWHSVL IERQGRSINL TLDRSMQHFR TNGEFDYLDL DYEITFGGIP FSGKPSSSNR KNFKGCMESI NYNGVNITDL ARRKKLGPSN MGNLSFSCVE PYTVPVFFNA TSYLEVPGRL NQDLFSVSFQ FRTWNPSGLL LFSHFADNLG NVEIDLVESK VGVHINNTQT KTSQIDISSG SGLNDGQWHE VRFLAKENFA VLTIDGDEAS AVRTNSPLQV KTGEKYFFGG FLNHMNNASY SALQPSFQGC MQLIQVDDQL VNLYEVAQRK PGSFANVTID MCAIIDRCVP NHCEHGGKCS QTWDSFKCTC DETGYSGATC HNSIYEPSCE AYKHLGQTSN YYWIDPDGSG PLGPLKVYCN MTEDKVWTIV SHDLQMQTTV VGYNPEKYSV TQLIYSASMD QISAITSSAE YCEQYVSYFC RMSRLLNTPD GSPYTWWVGK ANEKHYYWGG SEPGIQKCAC GIERNCTDPK YYCNCDADYK QWRKDAGFLS YKDHLPVSQV VVGDTDRQGS EAKLSVGPLR CQGDRNYWNA ASFPNPSSYL HFSTFQGETS ADISFYFKTL IPRGVFLENL GNTDFIKLEL KSATEVSFSF DVGNGPVEIV VRSPSPLNDD QWHRVTAERN VKQASLQVDR LPQQIRKAPT EGHTRLELYS QLFVGGAGGQ QGFLGCIRSL RMNGVTLDLE ERAKVTSGFK SGCSGHCTSY GANCENGGKC IEKYHGYSCD CSNTAYDGTF CNKDVGAFFE EGMWLRYNFQ APAVTARDTG SRAENSADQQ QHLAPDLAQE QIHFSFSTTK APCILLYVSS LTTDFLAVLV KPTGNLQIRY NLGGTREPFN IDVDHRNMAN GQPHSVNITR HERTIILKLD HYPAVGYHLP SSSDTLFNSP KSLFLGKVIE TGKIDQEIHK YNTPGFTGCL SRVQFNHIAP LKAALRQTNA SAHVHIQGEL VESNCGASPL TLSPMSSATD PWHLDHLDSA SADFPYNPGQ GQAIRNGVNR NS |
| 预测分子量 | 139 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CNTNAP2重组蛋白的参考文献示例(注：部分文献为示例性概括，实际引用时请核实具体来源)：
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1. **文献名称**：*Structural and Functional Analysis of the CNTNAP2 Extracellular Domain*
**作者**：Smith, J., et al.
**摘要**：本研究成功在大肠杆菌和哺乳动物细胞系统中表达并纯化了CNTNAP2的胞外结构域重组蛋白。通过X射线晶体学解析了其三维结构，揭示了该结构域中关键的Laminin G和Epidermal Growth Factor(EGF)重复模块，并证实其与突触配体Neurexin-1的结合能力，为神经细胞黏附机制提供了分子基础。
2. **文献名称**：*CNTNAP2 Recombinant Protein Restores Synaptic Deficits in a Mouse Model of Autism*
**作者**：Lee, S., et al.
**摘要**：通过昆虫细胞表达系统制备全长CNTNAP2重组蛋白，并在Cntnap2基因敲除小鼠模型中验证其功能。结果显示，重组蛋白可通过血脑屏障递送并部分恢复突触可塑性和社交行为缺陷，提示其作为潜在治疗工具的可行性。
3. **文献名称**：*Phosphorylation Dynamics of the CNTNAP2 Intracellular Domain Regulates Neuronal Morphogenesis*
**作者**：Garcia, R., et al.
**摘要**：利用HEK293细胞表达并纯化CNTNAP2胞内结构域重组蛋白，结合质谱分析鉴定了多个酪氨酸磷酸化位点。功能实验表明，磷酸化修饰通过调控与PDZ结构域蛋白的相互作用，影响神经元树突发育和轴突导向。
4. **文献名称**：*Interaction Screening Identifies Novel Binding Partners of CNTNAP2 in the Postsynaptic Density*
**作者**：Wang, Y., et al.
**摘要**：采用GST标签融合的CNTNAP2重组蛋白进行体外Pull-down实验，结合蛋白质组学技术发现其与突触后致密蛋白(如Shank3和DLGAP1)的直接结合。研究揭示了CNTNAP2在突触后信号复合体组装中的潜在作用。
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**备注**：以上文献为示例性内容，实际研究中请通过PubMed或Web of Science等数据库检索真实文献(如搜索关键词：CNTNAP2 recombinant protein, CNTNAP2 expression, CNTNAP2 structure/function)。已知与CNTNAP2相关的权威研究团队包括Daniel Geschwind、Elise Robinson等，可重点关注其发表的工作。
The CNTNAP2 (Contactin-Associated Protein-Like 2) gene encodes a member of the neurexin superfamily, which plays critical roles in neuronal development, synaptic function, and cell adhesion. As a transmembrane protein, CNTNAP2 features multiple extracellular domains, including laminin G and epidermal growth factor (EGF)-like repeats, which facilitate interactions with other proteins and extracellular matrix components. It is highly expressed in brain regions associated with language and cognition, influencing axon guidance, myelination, and the formation of neuronal networks.
Mutations or variations in CNTNAP2 are strongly linked to neurodevelopmental disorders such as autism spectrum disorder (ASD), intellectual disability, schizophrenia, and epilepsy. Notably, homozygous loss-of-function mutations cause a severe autosomal recessive condition called cortical dysplasia-focal epilepsy syndrome. These associations highlight CNTNAP2’s importance in maintaining neural circuit integrity.
Recombinant CNTNAP2 protein, produced via expression systems like mammalian or insect cells, retains functional domains for experimental studies. Purification methods, such as affinity tags (e.g., His-tag), ensure high specificity. This engineered protein is widely used to investigate CNTNAP2’s biochemical properties, ligand-receptor interactions, and pathological mechanisms. For example, it aids in mapping binding partners like contactin or neurexins, elucidating synaptic adhesion pathways. Additionally, recombinant CNTNAP2 serves as an antigen for antibody development, a substrate for drug screening, and a tool to model cellular deficits in neurodevelopmental disorders.
Research utilizing recombinant CNTNAP2 has advanced our understanding of its role in neural connectivity and disease, offering potential avenues for diagnostics or targeted therapies. Its applications bridge molecular insights with translational efforts in neuropsychiatry.
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