| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IGSF3 |
| Uniprot No | O75054 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1194aa |
| 氨基酸序列 | MKCFFPVLSCLAVLGVVSAQRQVTVQEGPLYRTEGSHITIWCNVSGYQGPSEQNFQWSIYLPSSPEREVQIVSTMDSSFPYAIYTQRVRGGKIFIERVQGNSTLLHITDLQARDAGEYECHTPSTDKQYFGSYSAKMNLVVIPDSLQTTAMPQTLHRVEQDPLELTCEVASETIQHSHLSVAWLRQKVGEKPVEVISLSRDFMLHSSSEYAQRQSLGEVRLDKLGRTTFRLTIFHLQPSDQGEFYCEAAEWIQDPDGSWYAMTRKRSEGAVVNVQPTDKEFTVRLETEKRLHTVGEPVEFRCILEAQNVPDRYFAVSWAFNSSLIATMGPNAVPVLNSEFAHREARGQLKVAKESDSVFVLKIYHLRQEDSGKYNCRVTEREKTVTGEFIDKESKRPKNIPIIVLPLKSSISVEVASNASVILEGEDLRFSCSVRTAGRPQGRFSVIWQLVDRQNRRSNIMWLDRDGTVQPGSSYWERSSFGGVQMEQVQPNSFSLGIFNSRKEDEGQYECHVTEWVRAVDGEWQIVGERRASTPISITALEMGFAVTAISRTPGVTYSDSFDLQCIIKPHYPAWVPVSVTWRFQPVGTVEFHDLVTFTRDGGVQWGDRSSSFRTRTAIEKAESSNNVRLSISRASDTEAGKYQCVAELWRKNYNNTWTRLAERTSNLLEIRVLQPVTKLQVSKSKRTLTLVENKPIQLNCSVKSQTSQNSHFAVLWYVHKPSDADGKLILKTTHNSAFEYGTYAEEEGLRARLQFERHVSGGLFSLTVQRAEVSDSGSYYCHVEEWLLSPNYAWYKLAEEVSGRTEVTVKQPDSRLRLSQAQGNLSVLETRQVQLECVVLNRTSITSQLMVEWFVWKPNHPERETVARLSRDATFHYGEQAAKNNLKGRLHLESPSPGVYRLFIQNVAVQDSGTYSCHVEEWLPSPSGMWYKRAEDTAGQTALTVMRPDASLQVDTVVPNATVSEKAAFQLDCSIVSRSSQDSRFAVAWYSLRTKAGGKRSSPGLEEQEEEREEEEEEDDDDDDDPTERTALLSVGPDAVFGPEGSPWEGRLRFQRLSPVLYRLTVLQASPQDTGNYSCHVEEWLPSPQKEWYRLTEEESAPIGIRVLDTSPTLQSIICSNDALFYFVFFYPFPIFGILIITILLVRFKSRNSSKNSDGKNGVPLLWIKEPHLNYSPTCLEPPVLSIHPGAID |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IGSF3重组蛋白的3篇参考文献的简要整理(注：以下内容为模拟示例，实际文献需通过学术数据库验证)：
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1. **文献名称**: *"IGSF3 regulates cell adhesion and migration through integrin signaling pathways"*
**作者**: Smith A, et al.
**摘要**: 本研究利用重组人IGSF3蛋白，揭示了其通过结合β1整合素调控细胞黏附和迁移的分子机制。实验表明，重组IGSF3能增强上皮细胞的黏附能力，并激活FAK/Src信号通路，提示其在组织修复中的潜在作用。
2. **文献名称**: *"Structural and functional analysis of recombinant IGSF3 in neurodevelopmental disorders"*
**作者**: Tanaka K, et al.
**摘要**: 通过在大肠杆菌中表达并纯化IGSF3胞外域重组蛋白，作者解析了其免疫球蛋白样结构域的三维构象。进一步实验发现，该重组蛋白可促进神经元突触形成，为研究IGSF3基因突变与神经发育异常的关联提供了依据。
3. **文献名称**: *"Recombinant IGSF3 as a potential biomarker in hepatocellular carcinoma"*
**作者**: Chen L, et al.
**摘要**: 研究团队在HEK293细胞中表达带有His标签的IGSF3重组蛋白，并发现肝癌患者血清中IGSF3水平显著升高。体外实验显示，重组IGSF3可通过抑制TGF-β通路减缓肿瘤细胞侵袭，提示其作为治疗靶点的可能性。
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**注意**：以上文献信息为模拟生成，具体研究需查阅真实数据库(如PubMed、Web of Science)。建议结合关键词“IGSF3 recombinant protein”或“IGSF3 expression and function”进一步检索最新成果。
IGSF3 (Immunoglobulin Superfamily Member 3) is a transmembrane protein belonging to the immunoglobulin superfamily, characterized by extracellular immunoglobulin (Ig) domains that mediate cell-cell interactions and intracellular signaling. It is encoded by the *IGSF3* gene, located on chromosome 1 in humans, and is expressed in various tissues, including the brain, testis, and immune cells. IGSF3 has been implicated in diverse physiological processes, such as neuronal development, spermatogenesis, and immune regulation. Structurally, it contains multiple Ig-like domains, a transmembrane region, and a cytoplasmic tail that may interact with intracellular signaling molecules.
Recombinant IGSF3 protein is engineered in vitro using expression systems like mammalian cells (e.g., HEK293) or insect cells to ensure proper folding and post-translational modifications. This purified protein serves as a critical tool for studying IGSF3’s biological functions, including its role in cell adhesion, receptor-ligand interactions, and signaling pathways. Research suggests IGSF3 may interact with proteins such as neurexins, influencing synaptic organization, or modulate immune cell communication through unidentified partners. Dysregulation of IGSF3 has been linked to neurodevelopmental disorders, autoimmune conditions, and cancer progression, highlighting its potential as a therapeutic target or biomarker.
Current studies utilize recombinant IGSF3 to investigate its molecular mechanisms, develop antibodies for diagnostic assays, and explore its involvement in diseases. For example, in neuroscience, it aids in mapping synaptic protein networks, while in oncology, it helps assess its role in tumor invasiveness. Despite progress, many aspects of IGSF3’s function remain unclear, driving ongoing research to elucidate its precise roles in health and disease. The availability of recombinant IGSF3 accelerates these efforts, offering insights into its therapeutic and diagnostic applications.
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