| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBG |
| Uniprot No | P05543 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-415aa |
| 氨基酸序列 | MSPFLYLVLLVLGLHATIHCASPEGKVTACHSSQPNATLYKMSSINADFA FNLYRRFTVETPDKNIFFSPVSISAALVMLSFGACCSTQTEIVETLGFNL TDTPMVEIQHGFQHLICSLNFPKKELELQIGNALFIGKHLKPLAKFLNDV KTLYETEVFSTDFSNISAAKQEINSHVEMQTKGKVVGLIQDLKPNTIMVL VNYIHFKAQWANPFDPSKTEDSSSFLIDKTTTVQVPMMHQMEQYYHLVDM ELNCTVLQMDYSKNALALFVLPKEGQMESVEAAMSSKTLKKWNRLLQKGW VDLFVPKFSISATYDLGATLLKMGIQHAYSENADFSGLTEDNGLKLSNAA HKAVLHIGEKGTEAAAVPEVELSDQPENTFLHPIIQIDRSFMLLILERST RSILFLGKVVNPTEA |
| 预测分子量 | 73 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TBG(甲状腺结合球蛋白)重组蛋白研究的3篇示例文献(内容为模拟概括，供参考)：
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1. **文献名称**: "High-yield expression and purification of recombinant human thyroxine-binding globulin in mammalian cells"
**作者**: Smith J, et al.
**摘要**: 报道了在HEK293细胞中高效表达重组人TBG的方法，通过优化载体设计和纯化流程获得高纯度蛋白，并验证其与甲状腺激素(T4/T3)的结合活性。
2. **文献名称**: "Structural analysis of recombinant TBG reveals key residues for hormone interaction"
**作者**: Chen L, et al.
**摘要**: 利用X射线晶体学解析了重组TBG的分子结构，揭示了其与甲状腺激素结合的关键氨基酸位点，为遗传性TBG缺陷症的机制研究提供依据。
3. **文献名称**: "Recombinant TBG as a diagnostic tool for thyroid function testing"
**作者**: Wang Y, et al.
**摘要**: 开发基于重组TBG的体外检测体系，证明其在甲状腺功能异常患者血清激素水平测定中的高灵敏度和特异性，优于传统检测方法。
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**备注**：以上为示例文献，实际文献请通过PubMed、Web of Science等数据库，以关键词"recombinant thyroxine-binding globulin"或"recombinant TBG"检索近年研究。
**Background of Recombinant TBG Protein**
Thyroxine-binding globulin (TBG) is a glycoprotein primarily synthesized in the liver and serves as the main transport carrier for thyroid hormones (THs), including thyroxine (T4) and triiodothyronine (T3), in the bloodstream. By binding over 70% of circulating THs, TBG regulates hormone bioavailability, ensuring stable systemic distribution and protecting hormones from rapid renal clearance. Its role in maintaining thyroid hormone homeostasis is critical for metabolic regulation, growth, and development.
Recombinant TBG (rTBG) is produced using genetic engineering techniques, typically by expressing the *SERPINA7* gene (encoding TBG) in mammalian cell lines (e.g., CHO or HEK293) or yeast systems. These platforms enable post-translational modifications, such as glycosylation, which are essential for TBG’s structural stability and hormone-binding capacity. Purification via affinity chromatography ensures high purity and functionality.
The development of rTBG addresses limitations of native TBG isolated from human plasma, including batch variability, ethical concerns, and pathogen risks. It is widely utilized in diagnostic assays to measure free thyroid hormones, aiding in the evaluation of thyroid disorders (e.g., hyperthyroidism, hypothyroidism). Researchers also employ rTBG to study hormone-protein interactions, genetic mutations causing TBG deficiencies or excess (e.g., TBG polymorphisms), and drug-TBG interactions that alter hormone pharmacokinetics. Additionally, rTBG serves as a reference material in calibrating immunoassays, improving diagnostic accuracy.
In drug development, rTBG helps assess how therapeutics affect TH distribution, particularly for agents targeting thyroid function or hormone replacement therapies. Its standardized production ensures reproducibility, making it valuable for both clinical and research applications. Overall, recombinant TBG represents a safe, scalable, and consistent tool for advancing thyroid-related diagnostics and therapeutics.
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