纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | H1F0 |
Uniprot No | P07305 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 2-194aa |
氨基酸序列 | MHHHHHHTENSTSAPAAKPKRAKASKKSTDHPKYSDMIVAAIQAEKNRAG SSRQSIQKYIKSHYKVGENADSQIKLSIKRLVTTGVLKQTKGVGASGSFR LAKSDEPKKSVAFKKTKKEIKKVATPKKASKPKKAASKAPTKKPKATPVK KAKKKLAATPKKAKKPKTVKAKPVKASKPKKAKPVKPKAKSSAKRAGKKK |
预测分子量 | 22 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于H1F0重组蛋白的示例参考文献(内容为模拟生成,仅供参考):
---
1. **文献名称**: *Structural insights into the H1F0 C-terminal domain and its interaction with DNA*
**作者**: Saeki, K. et al.
**摘要**: 通过X射线晶体学解析了重组H1F0蛋白C端结构域的构象,揭示了其与核小体DNA结合的分子机制,表明H1F0在染色质高级结构中发挥特异性调控作用。
2. **文献名称**: *H1F0重组蛋白在肿瘤细胞分化中的功能研究*
**作者**: Dworkin, L. & Brown, D.T.
**摘要**: 发现H1F0重组蛋白通过调控靶基因甲基化水平抑制肿瘤细胞增殖,实验表明其过表达可诱导癌细胞向终末分化表型转变。
3. **文献名称**: *Recombinant H1F0 as a novel gene delivery carrier in vitro*
**作者**: Torres, A. et al.
**摘要**: 验证了重组H1F0蛋白与质粒DNA的高效结合能力,开发了一种基于组蛋白的非病毒基因递送系统,显著提升细胞转染效率。
4. **文献名称**: *H1F0重组蛋白在表观遗传重编程中的应用*
**作者**: Harshman, S.W. et al.
**摘要**: 利用重组H1F0蛋白替换体细胞核中的连接组蛋白,成功诱导多能干细胞生成,证实其在重编程过程中对染色质开放性的调控作用。
---
**提示**:以上为模拟文献,实际文献需通过PubMed/Google Scholar检索关键词(如“H1F0 recombinant”“H1 histone variant”)获取。建议结合具体研究方向筛选高引论文或近期研究。
Histone H1F0. also known as H1.0 or H1°, is a linker histone protein that plays a critical role in chromatin organization and gene regulation. As a member of the H1 histone family, it binds to the nucleosome core particle, stabilizing higher-order chromatin structures and influencing DNA accessibility. Unlike replication-dependent H1 variants, H1F0 is constitutively expressed in most terminally differentiated cells, such as neurons and macrophages, and is associated with transcriptional repression in quiescent or senescent cells. Its expression is dynamically regulated during cellular differentiation, suggesting a role in maintaining cell identity and silencing pluripotency genes.
Recombinant H1F0 is produced using biotechnological methods, typically through expression in bacterial (e.g., E. coli) or eukaryotic systems, followed by purification via affinity chromatography. This engineered protein retains the conserved globular domain responsible for DNA binding and the disordered C-terminal tail involved in chromatin compaction. Researchers utilize recombinant H1F0 to study chromatin remodeling mechanisms, epigenetic regulation, and cellular differentiation processes. Its involvement in stabilizing heterochromatin makes it particularly relevant in cancer biology, where altered H1F0 expression has been observed in certain malignancies. Additionally, it serves as a tool for investigating nuclear reprogramming in stem cell research. Recent studies also explore its potential as a biomarker for cellular senescence and as a therapeutic target in age-related diseases. The recombinant form enables precise biochemical studies that are challenging with native H1F0 due to its tight chromatin association and low solubility in physiological conditions.
×