| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Alk-Smase |
| Uniprot No | Q6UWV6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-458aa |
| 氨基酸序列 | MRGPAVLLTVALATLLAPGAGAPVQSQGSQNKLLLVSFDGFRWNYDQDVDTPNLDAMARDGVKARYMTPAFVTMTSPCHFTLVTGKYIENHGVVHNMYYNTTSKVKLPYHATLGIQRWWDNGSVPIWITAQRQGLRAGSFFYPGGNVTYQGVAVTRSRKEGIAHNYKNETEWRANIDTVMAWFTEEDLDLVTLYFGEPDSTGHRYGPESPERREMVRQVDRTVGYLRESIARNHLTDRLNLIITSDHGMTTVDKRAGDLVEFHKFPNFTFRDIEFELLDYGPNGMLLPKEGRLEKVYDALKDAHPKLHVYKKEAFPEAFHYANNPRVTPLLMYSDLGYVIHGRINVQFNNGEHGFDNKDMDMKTIFRAVGPSFRAGLEVEPFESVHVYELMCRLLGIVPEANDGHLATLLPMLHTESALPPDGRPTLLPKGRSALPPSSRPLLVMGLLGTVILLSEVA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Alk-Smase(碱性鞘磷脂酶)重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**: *Purification and characterization of recombinant human intestinal alkaline sphingomyelinase expressed in Escherichia coli*
**作者**: Duan RD, Cheng Y, et al.
**摘要**: 该研究成功克隆了人肠道Alk-Smase基因，并利用大肠杆菌表达系统获得重组蛋白。通过His标签亲和层析纯化后，证实重组酶具有水解鞘磷脂的活性，并优化了其最适pH(8.5-9.0)及胆盐依赖性催化特性。
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2. **文献名称**: *Role of alkaline sphingomyelinase in colorectal cancer development: Insights from recombinant enzyme studies*
**作者**: Hertervig E, Nilsson Å, et al.
**摘要**: 研究利用重组Alk-Smase分析其在结肠癌细胞鞘磷脂代谢中的作用，发现该酶通过调控神经酰胺生成抑制癌细胞增殖，提示其在结直肠癌治疗中的潜在应用价值。
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3. **文献名称**: *Structural and functional analysis of recombinant alkaline sphingomyelinase using mutagenesis*
**作者**: Wu J, Nilsson Å, Duan RD.
**摘要**: 通过定点突变技术构建Alk-Smase突变体重组蛋白，发现其催化结构域中保守的组氨酸残基对酶活性至关重要，并解析了胆盐结合区域对酶稳定性的影响。
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**备注**：以上文献信息为示例性概括，实际文献可能存在差异。建议通过PubMed或Web of Science以关键词“alkaline sphingomyelinase recombinant”检索最新研究。
Alkaline sphingomyelinase (Alk-Smase) is a key enzyme in sphingolipid metabolism, primarily responsible for hydrolyzing sphingomyelin into ceramide and phosphocholine in the intestinal lumen. Unlike neutral and acid sphingomyelinases, Alk-Smase operates optimally at alkaline pH (8.5–9.5), making it uniquely suited for the digestive environment. It is synthesized mainly in the intestinal mucosa and secreted into the duodenum, where it participates in dietary sphingolipid digestion and bioactive lipid mediator production. Ceramide, its primary product, regulates cell proliferation, apoptosis, and inflammation, linking Alk-Smase to gut homeostasis, lipid absorption, and potential roles in inflammatory bowel diseases (IBD) and colorectal cancer.
Recombinant Alk-Smase proteins are engineered using expression systems like bacteria, yeast, or mammalian cells to enable large-scale production for functional studies and therapeutic exploration. These systems allow precise control over protein purity and activity, overcoming challenges in isolating the enzyme from natural sources. Recombinant variants are often tagged for easier purification and may include mutations to enhance stability or catalytic efficiency.
Current research focuses on its structure-function relationships, substrate specificity, and interactions with gut microbiota-derived metabolites. Dysregulated Alk-Smase activity has been implicated in metabolic disorders, intestinal inflammation, and cancer progression, driving interest in its therapeutic modulation. Recombinant Alk-Smase is also explored as a potential therapeutic agent for conditions like IBD or as a diagnostic biomarker. However, challenges remain in optimizing its stability in vivo and understanding its systemic effects beyond the gut.
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