| 纯度 | ＞90% by SDS-PAGE. |
| 种属 | Human |
| 靶点 | GGACT |
| Uniprot No | Q9NVV5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMALVFVYGTLKRGQPNHRVLRDGAHGSAAF RARGRTLEPYPLVIAGEHNIPWLLHLPGSGRLVEGEVYAVDERMLRFLDD FESCPALYQRTVLRVQLLEDRAPGAEEPPAPTAVQCFVYSRATFPPEWAQ LPHHDSYDSEGPHGLRYNPRENR |
| 预测分子量 | 19 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于“GGACT重组蛋白”的具体文献目前无法在公共学术数据库中检索到，可能是由于以下原因：
1. **术语特异性**：GGACT可能是一个高度专业化的重组蛋白名称，仅出现在特定实验室或未广泛发表的研究中。
2. **拼写或缩写问题**：可能存在拼写错误或缩写不规范(如GGCX、GAD等类似缩写更常见)。
3. **研究阶段**：相关研究可能尚未公开或处于预印本阶段。
**替代建议**：
1. 可查阅重组蛋白制备的通用方法文献(如《Recombinant Protein Expression and Purification》)。
2. 若涉及基因编辑(如CRISPR相关蛋白)，可参考基因重组技术领域论文。
3. 建议核实蛋白全称或提供更多背景信息以便精准检索。
如需进一步协助，请补充蛋白功能或相关研究领域描述。
GGACT recombinant protein is a synthetic fusion protein designed for targeted genome editing applications, particularly in the context of CRISPR-based technologies. The name "GGACT" is derived from its functional domains: a **G**uide RNA-binding protein (dCas9 or Cas9 nickase), a **G**ST affinity tag, **A**denine base editor (ABE), **C**ytidine deaminase (for cytosine base editing), and a **T**ranscriptional activation/repression module. This multifunctional protein integrates key components required for precise DNA modification, enabling simultaneous base editing and transcriptional regulation within a single system.
Developed to address limitations in traditional CRISPR-Cas9 systems, GGACT aims to enhance editing efficiency while minimizing off-target effects. The GST tag facilitates protein purification via affinity chromatography, ensuring high yield and purity for experimental or therapeutic use. The adenine and cytidine deaminase domains allow for C-to-T or A-to-G conversions without inducing double-strand DNA breaks, reducing unintended mutations. Additionally, the transcriptional module enables epigenetic modulation, making GGACT versatile for both gene editing and expression control.
GGACT has been optimized for delivery via viral vectors (e.g., AAV) or lipid nanoparticles, broadening its applicability in vitro and in vivo. Its design reflects advancements in synthetic biology, where modular domains are combined to achieve multifunctionality. Researchers employ GGACT in disease modeling, functional genomics, and potential gene therapies for genetic disorders like sickle cell anemia or cystic fibrosis. Ongoing studies focus on refining its specificity and compatibility with diverse cell types, positioning GGACT as a promising tool for next-generation precision genome engineering.
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