| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP6V1E1 |
| Uniprot No | P36543 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-226aa |
| 氨基酸序列 | MALSDADVQKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQTQRLKIMEYYEKKEKQIEQQKKIQMSNLMNQARLKVLRARDDLITDLLNEAKQRLSKVVKDTTRYQVLLDGLVLQGLYQLLEPRMIVRCRKQDFPLVKAAVQKAIPMYKIATKNDVDVQIDQESYLPEDIAGGVEIYNGDRKIKVSNTLESRLDLIAQQMMPEVRGALFGANANRKFLD |
| 分子量 | 50.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 冻干粉 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人V型质子ATP酶亚基E1(ATP6V1E1)的模拟参考文献及其摘要(基于领域常见研究方向虚构，仅供参考)：
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1. **文献名称**: *Structural insights into the human V-ATPase subunit ATP6V1E1 and its role in proton translocation*
**作者**: Lee S, et al.
**摘要**: 通过X射线晶体学解析ATP6V1E1的三维结构，揭示其与其他V-ATP酶亚基的相互作用界面，并证明该亚基在调控质子泵组装及pH稳态中的关键作用。
2. **文献名称**: *ATP6V1E1 overexpression promotes tumor metastasis via lysosomal acidification in breast cancer*
**作者**: Zhang Y, et al.
**摘要**: 研究表明，ATP6V1E1在乳腺癌细胞中高表达，通过增强V-ATP酶活性促进溶酶体酸化，从而激活组织蛋白酶释放，驱动肿瘤侵袭和转移。
3. **文献名称**: *Functional characterization of recombinant ATP6V1E1 mutations in osteopetrosis*
**作者**: Gupta R, et al.
**摘要**: 分析重组表达的ATP6V1E1突变体，发现特定位点变异(如R110L)会导致V-ATP酶功能丧失，破骨细胞酸化缺陷，进而诱发遗传性骨硬化症。
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*注：以上文献为模拟示例，实际研究中需通过PubMed或Web of Science等数据库检索真实论文。*
**Background of Recombinant Human V-Type Proton ATPase Subunit E1 (ATP6V1E1)**
The V-type proton ATPase (V-ATPase) is a multi-subunit enzyme critical for acidifying intracellular compartments, such as lysosomes and endosomes, by pumping protons across membranes. This process is essential for cellular functions like protein degradation, membrane trafficking, and signaling. The enzyme consists of two main domains: a cytosolic V1 sector (ATP hydrolysis) and a membrane-bound V0 sector (proton translocation).
ATP6V1E1 (V-ATPase subunit E1) is a key component of the V1 domain, specifically part of the E subunit family. This subunit exists as one of two isoforms (E1 and E2) encoded by distinct genes. ATP6V1E1 plays a structural and regulatory role in V-ATPase assembly and activity, impacting the enzyme’s stability and proton transport efficiency. Studies suggest it may also participate in extracellular acidification, influencing processes like bone resorption and tumor microenvironment modulation.
Dysregulation of ATP6V1E1 has been linked to diseases, including cancer (e.g., enhanced tumor invasion via pH-dependent pathways), osteoporosis (impaired osteoclast function), and renal tubular acidosis. Recombinant ATP6V1E1 is widely used in biochemical studies to dissect V-ATPase mechanisms, develop inhibitors, or explore therapeutic targets. Its recombinant form enables precise investigation of structure-function relationships and disease-associated mutations. Current research focuses on understanding its isoform-specific roles and potential as a biomarker or drug target in pH-dependent pathologies.
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