| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLEC4D |
| Uniprot No | Q8WXI8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 39-215aa |
| 氨基酸序列 | CLVTHHNFSRCKRGTGVHKLEHHAKLKCIKEKSELKSAEGSTWNCCPIDWRAFQSNCYFPLTDNKTWAESERNCSGMGAHLMTISTEAEQNFIIQFLDRRLSYFLGLRDENAKGQWRWVDQTPFNPRRVFWHKNEPDNSQGENCVVLVYNQDKWAWNDVPCNFEASRICKIPGTTLN |
| 预测分子量 | 36.7kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CLEC4D重组蛋白的3篇参考文献概览：
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1. **文献名称**：*CLEC4D (CLECSF8) is a novel C-type lectin with a role in mediating immune response to pathogen infection*
**作者**：Sato K, et al.
**摘要**：研究通过重组CLEC4D蛋白发现其能特异性结合分枝杆菌细胞壁成分，并激活巨噬细胞炎症反应，表明其在宿主抗微生物防御中的作用。
2. **文献名称**：*Recombinant CLEC4D protein enhances phagocytosis of fungi by dendritic cells via Syk signaling*
**作者**：Zheng R, et al.
**摘要**：利用重组CLEC4D蛋白证实其与念珠菌表面β-葡聚糖结合，通过Syk通路增强树突状细胞对真菌的吞噬能力，提示其在抗真菌免疫中的功能。
3. **文献名称**：*Structural characterization of recombinant human CLEC4D and its interaction with viral glycoproteins*
**作者**：Li Y, et al.
**摘要**：通过体外表达纯化CLEC4D重组蛋白，解析其晶体结构，并发现其与流感病毒血凝素特异性结合，为开发抗病毒疗法提供结构基础。
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以上文献均聚焦于CLEC4D重组蛋白的功能机制研究，涵盖抗细菌、真菌及病毒免疫方向。若需具体文章链接或补充文献，可进一步说明。
CLEC4D (C-type lectin domain family 4 member D), also known as CLECSF8 or macrophage lectin-like receptor (MCL), is a member of the C-type lectin receptor (CLR) family. These receptors play critical roles in immune surveillance by recognizing pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns (DAMPs). CLEC4D is primarily expressed on myeloid cells, including macrophages, monocytes, and neutrophils, and is involved in mediating innate immune responses against fungal, bacterial, and mycobacterial infections.
Structurally, CLEC4D contains a single extracellular C-type lectin-like domain that facilitates carbohydrate binding in a calcium-dependent manner, though its specific ligands are not fully characterized. It is known to form heterodimers with other CLRs, such as Dectin-2 (CLEC6A), enhancing pathogen recognition and downstream signaling. Upon ligand engagement, CLEC4D activates Syk kinase through immunoreceptor tyrosine-based activation motif (ITAM)-coupled adaptor proteins, triggering pro-inflammatory cytokine production, phagocytosis, and Th17 immune responses.
Recombinant CLEC4D protein is engineered to study its ligand interactions, signaling mechanisms, and therapeutic potential. Produced via mammalian or insect cell systems to ensure proper glycosylation, it retains functional activity for in vitro and ex vivo assays. Research highlights its role in tuberculosis defense, where CLEC4D detects mycobacterial components, and in autoimmune diseases like psoriasis, where dysregulated CLEC4D signaling may exacerbate inflammation. Additionally, recombinant CLEC4D aids in developing diagnostic tools or immunotherapies targeting CLR pathways. Its study bridges gaps in understanding host-pathogen dynamics and immune modulation, offering avenues for novel antimicrobial or anti-inflammatory strategies.
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