| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PKD2 |
| Uniprot No | Q9BZL6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-878aa |
| 氨基酸序列 | MATAPSYPAGLPGSPGPGSPPPPGGLELQSPPPLLPQIPAPGSGVSFHIQ IGLTREFVLLPAASELAHVKQLACSIVDQKFPECGFYGLYDKILLFKHDP TSANLLQLVRSSGDIQEGDLVEVVLSASATFEDFQIRPHALTVHSYRAPA FCDHCGEMLFGLVRQGLKCDGCGLNYHKRCAFSIPNNCSGARKRRLSSTS LASGHSVRLGTSESLPCTAEELSRSTTELLPRRPPSSSSSSSASSYTGRP IELDKMLLSKVKVPHTFLIHSYTRPTVCQACKKLLKGLFRQGLQCKDCKF NCHKRCATRVPNDCLGEALINGDVPMEEATDFSEADKSALMDESEDSGVI PGSHSENALHASEEEEGEGGKAQSSLGYIPLMRVVQSVRHTTRKSSTTLR EGWVVHYSNKDTLRKRHYWRLDCKCITLFQNNTTNRYYKEIPLSEILTVE SAQNFSLVPPGTNPHCFEIVTANATYFVGEMPGGTPGGPSGQGAEAARGW ETAIRQALMPVILQDAPSAPGHAPHRQASLSISVSNSQIQENVDIATVYQ IFPDEVLGSGQFGVVYGGKHRKTGRDVAVKVIDKLRFPTKQESQLRNEVA ILQSLRHPGIVNLECMFETPEKVFVVMEKLHGDMLEMILSSEKGRLPERL TKFLITQILVALRHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFAR IIGEKSFRRSVVGTPAYLAPEVLLNQGYNRSLDMWSVGVIMYVSLSGTFP FNEDEDINDQIQNAAFMYPASPWSHISAGAIDLINNLLQVKMRKRYSVDK SLSHPWLQEYQTWLDLRELEGKMGERYITHESDDARWEQFAAEHPLPGSG LPTDRDLGGACPPQDHDMQELAERISVL |
| 预测分子量 | 130 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PKD2重组蛋白的3-4篇参考文献及其摘要概括：
1. **"Co-assembly of polycystin-1 and -2 produces unique cation-permeable currents"**
- **作者**: Hanaoka, K. et al.
- **摘要**: 本研究在HEK293细胞中重组共表达PKD1和PKD2蛋白，发现两者形成复合物后产生非选择性阳离子通道活性，提示其可能通过调节钙信号通路参与常染色体显性多囊肾病的发病机制。
2. **"Functional reconstitution of human polycystin-2"**
- **作者**: Gonzales, P. A. et al.
- **摘要**: 通过重组表达纯化人源PKD2蛋白，并在人工脂质体中验证其钙激活的阳离子通道功能，揭示了PKD2在细胞膜上的独立通道活性及其对钙离子浓度的敏感性。
3. **"Structure of the human PKD1-PKD2 complex"**
- **作者**: Su, Q. et al.
- **摘要**: 利用冷冻电镜技术解析了PKD1和PKD2复合体的高分辨率结构，阐明了二者跨膜结构域的相互作用及自动抑制调控机制，为疾病相关突变的功能研究提供了结构基础。
4. **"Regulation of ryanodine receptor-dependent calcium signaling by polycystin-2"**
- **作者**: Anyatonwu, G. I. et al.
- **摘要**: 研究发现重组PKD2蛋白与兰尼碱受体(RyR)在内质网膜上相互作用，调控细胞内钙释放，表明PKD2可能通过影响钙稳态参与多囊肾病的病理过程。
这些研究涵盖了PKD2重组蛋白的共表达、功能验证、结构解析及其在钙信号中的调控作用，反映了不同层面的研究进展。
**Background of PKD2 Recombinant Protein**
The PKD2 gene, located on chromosome 4 (4q22.1), encodes polycystin-2 (PC2 or TRPP2), a member of the transient receptor potential (TRP) channel superfamily. This protein is a calcium-permeable non-selective cation channel involved in cellular calcium signaling and mechanosensation. Structurally, polycystin-2 contains six transmembrane domains with intracellular N- and C-termini, and it interacts with polycystin-1 (encoded by PKD1) to form a heteromeric complex. This complex localizes to primary cilia, endoplasmic reticulum, and plasma membrane, playing critical roles in maintaining renal tubular architecture, fluid-flow sensing, and cell cycle regulation.
Mutations in PKD2 are a primary cause of autosomal dominant polycystic kidney disease (ADPKD), a disorder characterized by progressive cyst formation in kidneys and other organs. ADPKD affects approximately 1 in 1.000 individuals and accounts for 10–15% of end-stage renal disease cases. PKD2-linked ADPKD typically exhibits milder renal pathology but earlier extrarenal manifestations (e.g., hypertension, liver cysts) compared to PKD1 mutations. Mechanistically, loss-of-function mutations disrupt calcium homeostasis, ciliary signaling, and tissue repair pathways, driving cystogenesis.
Recombinant PKD2 protein is widely used to study its biochemical properties, channel activity, and interactions. Produced via heterologous expression systems (e.g., mammalian, insect, or bacterial cells), it enables structural analyses (e.g., cryo-EM) to resolve channel gating mechanisms and mutation-induced dysfunctions. Researchers also employ recombinant PKD2 in drug screening platforms to identify modulators of polycystin signaling, aiming to develop therapies targeting ADPKD. Additionally, it serves as an antigen for antibody production in diagnostic assays. Despite challenges in purifying this large, membrane-associated protein, advances in solubilization and tagging techniques have enhanced its utility in dissecting the pathobiology of polycystic diseases and related ciliopathies.
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