| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HRG |
| Uniprot No | P04196 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-525aa |
| 氨基酸序列 | VSPTDCSAVEPEAEKALDLINKRRRDGYLFQLLRIADAHLDRVENTTVYY LVLDVQESDCSVLSRKYWNDCEPPDSRRPSEIVIGQCKVIATRHSHESQD LRVIDFNCTTSSVSSALANTKDSPVLIDFFEDTERYRKQANKALEKYKEE NDDFASFRVDRIERVARVRGGEGTGYFVDFSVRNCPRHHFPRHPNVFGFC RADLFYDVEALDLESPKNLVINCEVFDPQEHENINGVPPHLGHPFHWGGH ERSSTTKPPFKPHGSRDHHHPHKPHEHGPPPPPDERDHSHGPPLPQGPPP LLPMSCSSCQHATFGTNGAQRHSHNNNSSDLHPHKHHSHEQHPHGHHPHA HHPHEHDTHRQHPHGHHPHGHHPHGHHPHGHHPHGHHPHCHDFQDYGPCD PPPHNQGHCCHGHGPPPGHLRRRGPGKGPRPFHCRQIGSVYRLPPLRKGE VLPLPEANFPSFPLPHHKHPLKPDNQPFPQSVSESCPGKFKSGFPQVSMF FTHTFPK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于HRG(Heregulin/Neuregulin)重组蛋白的文献摘要概览(基于公开研究整理)：
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1. **文献名称**: "Recombinant Heregulin-Pseudomonas Exotoxin Fusion Proteins: Pharmacokinetics and Toxicology"
**作者**: Liu C. et al.
**摘要**: 研究重组HRG与假单胞菌外毒素融合蛋白的制备及抗肿瘤效果，证明其在体外可靶向抑制HER2过表达的乳腺癌细胞增殖，并评估了动物模型中的药代动力学和毒性。
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2. **文献名称**: "Neuregulin-1β recombinant protein (rhNRG-1) promotes cardiomyocyte repair after myocardial infarction"
**作者**: Bersell K. et al.
**摘要**: 探讨重组人神经调节蛋白-1β(rhNRG-1)通过激活ErbB受体通路，促进心肌细胞增殖和修复，为心肌梗死后心脏再生治疗提供实验依据。
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3. **文献名称**: "Structural and functional analysis of the Heregulin-ErbB3 receptor interaction"
**作者**: Jones J.T. et al.
**摘要**: 通过重组HRG蛋白解析其与ErbB3受体的结合机制，发现特定结构域对信号传导的关键作用，为靶向癌症治疗的抗体设计提供结构基础。
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4. **文献名称**: "Recombinant neuregulin-1 enhances synaptic plasticity in a rodent model of Alzheimer’s disease"
**作者**: Li B. et al.
**摘要**: 研究显示，重组神经调节蛋白-1可通过调节NMDA受体活性改善阿尔茨海默病模型动物的突触可塑性和认知功能，提示其神经保护潜力。
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**注**：以上文献信息为示例性质，实际研究中建议通过PubMed或Web of Science检索最新文献(可结合关键词：Heregulin/Neuregulin, recombinant protein, ErbB signaling)。
Here’s a concise overview of HRG recombinant protein:
Histidine-rich glycoprotein (HRG) is a multifunctional plasma protein first identified in the 1970s. It is synthesized primarily in the liver and circulates at concentrations of 50-150 μg/mL in human blood. Structurally, HRG contains two N-terminal cystatin-like domains, a histidine/proline-rich (HRP) region, and a C-terminal domain, with its functional versatility stemming from interactions through these domains.
HRG plays regulatory roles in various physiological processes, including immune modulation, angiogenesis, apoptosis, and coagulation. It binds diverse ligands such as heparin, tropomyosin, fibrinogen, plasminogen, and microbial components, acting as a molecular adaptor that bridges cellular and extracellular components. Notably, HRG influences macrophage polarization, inhibits excessive blood vessel formation through interactions with vascular endothelial growth factor (VEGF), and modulates thrombus formation by regulating fibrinogen-clot structure.
Recombinant HRG production, achieved through bacterial or mammalian expression systems, has enabled detailed functional studies and therapeutic exploration. Its potential applications span cancer therapy (inhibiting tumor angiogenesis and metastasis), cardiovascular disease management (regulating thrombosis), and anti-inflammatory treatments. Recent research highlights HRG's diagnostic potential as a biomarker for conditions like sepsis and preeclampsia, where altered plasma levels correlate with disease progression.
Challenges in HRG research include maintaining proper post-translational modifications during recombinant production and understanding its context-dependent functions. Current studies focus on optimizing recombinant variants for therapeutic use and elucidating its role in tumor microenvironments. As a naturally occurring regulatory protein, HRG represents a promising candidate for developing multifunctional biologics with applications across immunology, hematology, and oncology.
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