以下是关于NUP50重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**: *Nup50 is required for cell cycle progression and chromatin binding of RanGAP1 in Xenopus laevis*
**作者**: Lindsay, C.R. et al.
**摘要**: 该研究通过体外重组表达Xenopus的NUP50蛋白，发现其与RanGAP1的相互作用对细胞周期调控至关重要，并揭示了NUP50在核膜重组和纺锤体组装中的功能机制。
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2. **文献名称**: *Structural and functional analysis of the C-terminal domain of Nup50 in nuclear pore complex assembly*
**作者**: Hattersley, N. et al.
**摘要**: 作者利用重组人源NUP50的C端结构域进行结构解析(X射线晶体学)，发现其通过结合Importin-α调控核转运，并证明该结构域在核孔复合体动态组装中的关键作用。
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3. **文献名称**: *Nup50 interacts with Sox2 to regulate neural stem cell differentiation via chromatin remodeling*
**作者**: Li, Y. et al.
**摘要**: 通过重组NUP50蛋白与Sox2的体外互作实验，研究揭示了NUP50通过调控染色质可及性影响神经干细胞分化，并提出了其在表观遗传调控中的非经典功能。
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**备注**：以上文献信息为基于NUP50相关研究的模拟概括，实际文献需通过PubMed/Google Scholar检索确认。若需具体文献DOI或发表年份，可进一步补充关键词优化检索。
NUP50 (nucleoporin 50) is a key component of the nuclear pore complex (NPC), a large protein assembly that regulates nucleocytoplasmic transport in eukaryotic cells. As a member of the nucleoporin family, NUP50 localizes to the nuclear side of the NPC and plays critical roles in both nuclear import and export processes. Structurally, it contains characteristic phenylalanine-glycine (FG) repeats that facilitate transient interactions with transport receptors, such as Importin-α and CRM1. to mediate the passage of cargo molecules. Unlike many nucleoporins, NUP50 dynamically shuttles between the NPC and nucleoplasm, suggesting additional regulatory functions beyond passive diffusion barriers.
Research highlights its involvement in mRNA export, mitotic progression, and chromatin organization. During mitosis, NUP50 participates in spindle assembly and chromosome segregation by interacting with microtubule-associated proteins. Its dysregulation has been linked to genomic instability and cancer progression. Recombinant NUP50 protein, typically produced in bacterial or mammalian expression systems with affinity tags (e.g., GST or His-tags), enables in vitro studies of NPC architecture, transport mechanisms, and protein interaction networks. Purified NUP50 is used to investigate its binding partners, post-translational modifications, and role in diseases. Recent studies also explore its potential as a biomarker or therapeutic target in oncology, particularly in cancers with altered nuclear transport machinery. Advances in cryo-EM and structural biology have further clarified its conformational dynamics within the NPC scaffold.
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