| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FPR3 |
| Uniprot No | P25089 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 254-353aa |
| 氨基酸序列 | WFPYELIGILMAVWLKEMLLNGKYKIILVLINPTSSLAFFNSCLNPILYVFMGRNFQERLIRSLPTSLERALTEVPDSAQTSNTDTTSASPPEETELQAM |
| 分子量 | 36.63 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FPR3蛋白的假设性参考文献示例(供参考，实际文献需通过学术数据库验证)：
1. **文献名称**：Expression and functional characterization of recombinant human FPR3 in neutrophil activation
**作者**：Smith A, et al.
**摘要**：研究成功在HEK293细胞中表达重组人FPR3蛋白，证实其与配体结合后激活MAPK信号通路，并促进中性粒细胞趋化性和炎性因子释放，提示FPR3在天然免疫中的作用。
2. **文献名称**：Structural insights into FPR3 ligand specificity through recombinant protein mutagenesis
**作者**：Chen L, et al.
**摘要**：通过重组FPR3的晶体结构解析和突变分析，发现其第2、3跨膜区残基对配体(如Annexin A1)选择性结合至关重要，为靶向药物设计提供结构基础。
3. **文献名称**：FPR3 mediates Aβ42-induced neuroinflammation via recombinant receptor reconstitution
**作者**：Wang Y, et al.
**摘要**：利用重组FPR3在胶质细胞中的过表达模型，证明其通过结合β淀粉样蛋白激活NLRP3炎症小体，可能参与阿尔茨海默病的神经炎症机制。
4. **文献名称**：High-yield production of soluble recombinant human FPR3 in Escherichia coli
**作者**：Zhang R, et al.
**摘要**：优化大肠杆菌表达系统实现可溶性FPR3的高效表达，通过亲和层析获得高纯度蛋白，适用于抗体开发及体外筛选研究。
**提示**：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索关键词(如"recombinant FPR3"、"FPR3 expression")。近期研究可能聚焦于FPR3与疾病(如癌症转移、炎症性疾病)的关联及治疗潜力。
**Background of Recombinant Human FPR3 Protein**
Formyl peptide receptor 3 (FPR3), a member of the G protein-coupled receptor (GPCR) family, is primarily involved in mediating immune and inflammatory responses. It is activated by pathogen- or host-derived formylated peptides, which act as chemoattractants to recruit and activate neutrophils, macrophages, and other immune cells. FPR3 plays a role in pathogen detection, phagocytosis, and the regulation of inflammation, though its exact biological functions remain less defined compared to other FPR family members (e.g., FPR1 and FPR2).
Recombinant human FPR3 protein is engineered using molecular cloning and expression systems (e.g., mammalian or insect cells) to produce a purified, bioactive form of the receptor. This allows researchers to study its structure, ligand-binding properties, and signaling mechanisms in vitro. FPR3 is implicated in diverse physiological and pathological processes, including infection, cancer progression, and autoimmune diseases. Its dual role in pro-resolving and pro-inflammatory pathways highlights its therapeutic potential as a drug target. Current research focuses on identifying specific ligands, dissecting downstream signaling pathways, and exploring its modulation in diseases like sepsis, rheumatoid arthritis, and neurodegenerative disorders. The development of recombinant FPR3 has accelerated structural studies and high-throughput screening for novel therapeutics.
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