| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPKOW |
| Uniprot No | Q92917 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-476aa |
| 氨基酸序列 | MADSKEGVLPLTAASTAPISFGFTRTSARRRLADSGDGAGPSPEEKDFLKTVEGRELQSVKPQEAPKELVIPLIQNGHRRQPPARPPGPSTDTGALADGVVSQAVKELIAESKKSLEERENAGVDPTLAIPMIQKGCTPSGEGADSEPRAETVPEEANYEAVPVEAYGLAMLRGMGWKPGEGIGRTFNQVVKPRVNSLRPKGLGLGANLTEAQALTPTGPSRMPRPDEEQEKDKEDQPQGLVPGGAVVVLSGPHRGLYGKVEGLDPDNVRAMVRLAVGSRVVTVSEYYLRPVSQQEFDKNTLDLRQQNGTASSRKTLWNQELYIQQDNSERKRKHLPDRQDGPAAKSEKAAPRSQHWLHRDLRVRFVDNMYKGGQYYNTKMIIEDVLSPDTCVCRTDEGRVLEGLREDMLETLVPKAEGDRVMVVLGPQTGRVGHLLSRDRARSRALVQLPRENQVVELHYDAICQYMGPSDTDDD |
| 分子量 | 78.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人GPKOW蛋白的参考文献示例(内容为虚构，仅供格式参考)：
1. **文献名称**：*Functional analysis of recombinant human GPKOW in mRNA splicing*
**作者**：Zhang L, Chen Y, Wang X
**摘要**：研究通过重组表达纯化人源GPKOW蛋白，发现其通过与U2 snRNP相互作用参与pre-mRNA剪接调控，验证了其在哺乳动物细胞中的剪接增强活性。
2. **文献名称**：*Structural characterization of GPKOW and its interaction with RNA polymerase II*
**作者**：Kim S, Lee JH, Park M
**摘要**：利用重组GPKOW蛋白进行晶体结构解析，揭示了其KOW结构域在结合RNA聚合酶ⅡCTD磷酸化位点的分子机制，提出其在转录延伸中的潜在作用。
3. **文献名称**：*Recombinant GPKOW production in E. coli and application in antiviral response studies*
**作者**：Guo R, Liu T, Xu H
**摘要**：报道在大肠杆菌中高效表达重组人GPKOW蛋白的优化方法，并证明其可增强宿主细胞对RNA病毒感染的免疫应答，提示其与干扰素信号通路的关联。
4. **文献名称**：*GPKOW knockdown and rescue assays using recombinant protein in neuronal cells*
**作者**：Müller F, Schmidt B
**摘要**：利用重组GPKOW蛋白进行神经元细胞回补实验，发现其可逆转基因敲低导致的突触形成缺陷，表明其在神经发育中的关键调控功能。
(注：以上文献为模拟内容，实际引用请查询真实数据库。)
Recombinant human GPKOW protein, also known as G-patch domain and KOW motifs-containing protein (GPATCH11 or KLP), is a nucleic acid-binding protein involved in RNA processing and transcriptional regulation. Originally identified through homology studies of RNA-associated proteins, GPKOW contains a conserved G-patch domain (implicated in RNA binding or protein interactions) and KOW motifs (common in ribosomal and spliceosomal proteins), suggesting roles in RNA metabolism. Studies highlight its interaction with RNA polymerase II and spliceosome components, linking it to pre-mRNA splicing, transcription elongation, and viral RNA response pathways, including hepatitis C and HIV replication.
Recombinant GPKOW is typically produced using *E. coli* or mammalian expression systems, enabling functional studies in vitro. Its purification often involves affinity tags (e.g., His-tag) followed by chromatography. Researchers utilize recombinant GPKOW to dissect its molecular mechanisms in RNA processing, investigate its role in viral pathogenesis, and explore potential links to diseases like cancer or neurological disorders due to spliceosome dysfunction. Recent work also suggests regulatory crosstalk between GPKOW and innate immune signaling, expanding its relevance to antiviral therapeutics. This protein serves as a critical tool for understanding RNA-protein dynamics in health and disease. (Word count: 238)
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