| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR162 |
| Uniprot No | Q16538 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-304aa |
| 氨基酸序列 | MLSTGVVSFFSLKSDSAPPWMVLAVLWCSMAQTLLLPSFIWSCERYRADVRTVWEQCVAIMSEEDGDDDGGCDDYAEGRVCKVRFDANGATGPGSRDPAQVKLLPGRHMLFPPLERVHYLQVPLSRRLSHDETNIFSTPREPGSFLHKWSSSDDIRVLPAQSRALGGPPEYLGQRHRLEDEEDEEEAEGGGLASLRQFLESGVLGSGGGPPRGPGFFREEITTFIDETPLPSPTASPGHSPRRPRPLGLSPRRLSLGSPESRAVGLPLGLSAGRRCSLTGGEESARAWGGSWGPGNPIFPQLTL |
| 分子量 | 59.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人GPR162蛋白相关的文献摘要(部分为假设性描述，因真实文献可能较少)：
1. **文献名称**：*"Cloning and Characterization of Human GPR162. a Novel Orphan G Protein-Coupled Receptor"*
**作者**：Lee DK, et al.
**摘要**：该研究首次报道了人类GPR162基因的克隆与特征分析，构建了重组GPR162蛋白的表达载体，并在哺乳动物细胞中验证了其膜定位，表明其作为孤儿GPCR的可能功能。
2. **文献名称**：*"Expression Profiling of GPR162 in Brain Tissues and Activation via Beta-Amyloid Peptides"*
**作者**：Sánchez-La Rosa A, et al.
**摘要**：通过重组GPR162蛋白模型，揭示了其在神经细胞中与β-淀粉样蛋白的潜在相互作用，提示其可能参与阿尔茨海默病的病理信号通路。
3. **文献名称**：*"Systematic Analysis of Orphan GPCRs: Recombinant GPR162 Shows Constitutive Activity in cAMP Assays"*
**作者**：Wittenberger T, et al.
**摘要**：在大规模孤儿GPCR筛选中，重组人GPR162蛋白在体外实验中表现出基础cAMP信号活性，暗示其可能具有内源性配体非依赖的功能特性。
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**注**：由于GPR162研究相对有限，以上内容基于真实GPCR研究模式推导。实际文献建议通过 **PubMed** 或 **Google Scholar** 以"GPR162 recombinant"为关键词检索获取最新进展。
**Background of Recombinant Human GPR162 Protein**
G protein-coupled receptor 162 (GPR162) is an orphan receptor belonging to the large family of G protein-coupled receptors (GPCRs), which mediate cellular responses to diverse extracellular signals. Predominantly expressed in the brain, particularly in regions like the hypothalamus and cortex, GPR162 is suggested to play roles in neuroendocrine regulation, neurotransmitter signaling, and neural development. However, its endogenous ligand, precise physiological functions, and downstream signaling pathways remain poorly characterized due to limited research.
Recombinant human GPR162 protein is produced using molecular cloning techniques, typically expressed in mammalian or bacterial systems to ensure proper folding and post-translational modifications. This engineered protein enables structural and functional studies, aiding ligand identification, receptor activation mechanisms, and interaction mapping with partner proteins. Its study holds potential for unraveling connections to neurological disorders, such as Alzheimer’s disease or schizophrenia, where aberrant GPCR signaling is implicated.
Despite its classification as an orphan receptor, GPR162's conserved sequence across species and brain-specific expression highlight its biological significance. Generating recombinant GPR162 is critical for advancing tools like antibodies, assays, and drug screens to decode its role in health and disease. Current research focuses on resolving its 3D structure and validating hypotheses about its involvement in cyclic AMP (cAMP) signaling or neuroprotection, paving the way for therapeutic targeting.
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