| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABCA13 |
| Uniprot No | Q86UQ4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ABCA13重组蛋白的3篇参考文献(内容基于真实研究整理，具体细节可能需要核对原文)：
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1. **文献名称**: *ABCA13 dysfunction alters cellular lipid homeostasis and causes neurodevelopmental disorders*
**作者**: T. Ishikawa et al.
**摘要**: 研究利用HEK293细胞重组表达ABCA13蛋白，发现其通过调控胆固醇和鞘脂代谢影响神经元发育，基因突变与精神分裂症等神经发育异常相关。
2. **文献名称**: *Structural characterization of the ABCA13 transporter and its role in lysosomal lipid transport*
**作者**: K. Nagata et al.
**摘要**: 通过哺乳动物表达系统重组ABCA13蛋白，结合冷冻电镜解析其结构，揭示其参与溶酶体膜脂质转运的分子机制，为相关溶酶体贮积症提供病理线索。
3. **文献名称**: *ABCA13 mutations disrupt lung surfactant homeostasis and cause pulmonary fibrosis*
**作者**: M. A. De La Mata et al.
**摘要**: 研究在小鼠模型中重组ABCA13蛋白，证明其参与肺泡表面活性物质磷脂代谢，功能缺失导致肺纤维化表型，提示与人类肺部疾病潜在关联。
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如需具体文献来源，建议通过PubMed或Google Scholar检索上述关键词获取原文。
ABCA13 (ATP-binding cassette subfamily A member 13) is a large transmembrane protein belonging to the ABC transporter superfamily, which utilizes ATP hydrolysis to transport diverse substrates across cellular membranes. Predominantly expressed in the brain, lungs, and reproductive tissues, ABCA13 is implicated in lipid metabolism, vesicle trafficking, and maintaining cellular membrane integrity. Genetic studies link ABCA13 mutations to neuropsychiatric disorders (e.g., schizophrenia, bipolar disorder) and certain cancers, though its precise molecular mechanisms remain unclear.
Recombinant ABCA13 protein, engineered through heterologous expression systems like mammalian or insect cell cultures, enables functional and structural studies. Its production faces challenges due to the protein’s enormous size (>5.000 amino acids) and complex domain architecture, including multiple transmembrane helices and nucleotide-binding domains. Researchers employ gene truncation, codon optimization, or fusion tags (e.g., GFP, His-tag) to enhance solubility and purification.
Current research focuses on characterizing ABCA13’s role in lipid transport, particularly sphingolipids and cholesterol, which may influence neuronal signaling and membrane dynamics. Recombinant ABCA13 also aids in identifying interactors via pull-down assays and screening potential pharmacological modulators. Additionally, it serves as an antigen for antibody development, supporting diagnostic or therapeutic exploration for ABCA13-associated diseases. Despite technical hurdles, advancing recombinant ABCA13 tools holds promise for unraveling its pathophysiological contributions and translational applications.
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