| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GRCC9 |
| Uniprot No | Q99619 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-263aa |
| 氨基酸序列 | MGQTALAGGSSSTPTPQALYPDLSCPEGLEELLSAPPPDLGAQRRHGWNPKDCSENIEVKEGGLYFERRPVAQSTDGARGKRGYSRGLHAWEISWPLEQRGTHAVVGVATALAPLQTDHYAALLGSNSESWGWDIGRGKLYHQSKGPGAPQYPAGTQGEQLEVPERLLVVLDMEEGTLGYAIGGTYLGPAFRGLKGRTLYPAVSAVWGQCQVRIRYLGERRAEPHSLLHLSRLCVRHNLGDTRLGQVSALPLPPAMKRYLLYQ |
| 分子量 | 55 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GRCC9蛋白的虚构参考文献示例(实际研究中若需真实文献，请通过学术数据库检索)：
---
1. **文献名称**: "Structural Insights into Human GRCC9 Protein through Cryo-EM Analysis"
**作者**: Zhang, L. et al.
**摘要**: 本研究利用冷冻电镜技术解析了重组人GRCC9蛋白的三维结构，发现其C端结构域具有独特的α-螺旋构象，可能与DNA结合功能相关。实验表明，该蛋白在体外可调节特定基因启动子活性，为GRCC9在转录调控中的作用提供了结构基础。
2. **文献名称**: "GRCC9 Knockout Reveals Its Role in Cancer Cell Apoptosis"
**作者**: Smith, J.R., Wang, Y.
**摘要**: 通过CRISPR/Cas9技术敲除GRCC9基因，发现其在结肠癌细胞中显著抑制 caspase-3 激活，导致凋亡受阻。重组人GRCC9蛋白的补充实验证实其通过p53通路调控肿瘤细胞死亡，提示其作为癌症治疗靶点的潜力。
3. **文献名称**: "Recombinant GRCC9 Protein Binds to Wnt/β-catenin Signaling Components"
**作者**: Li, H. et al.
**摘要**: 研究通过免疫共沉淀和体外Pull-down实验证明，重组人GRCC9蛋白直接与β-catenin相互作用，抑制Wnt信号通路活化。在斑马鱼模型中，GRCC9过表达导致胚胎发育异常，提示其参与发育调控及疾病机制。
4. **文献名称**: "Development of a GRCC9-Based Biosensor for Neurodegenerative Disease Biomarker Detection"
**作者**: Brown, K. & Patel, S.
**摘要**: 该团队利用重组人GRCC9蛋白的高亲和力特性，构建了一种电化学生物传感器，可检测脑脊液中τ蛋白的病理聚集形态。实验证明其在阿尔茨海默病早期诊断中灵敏度达95%，具有临床应用前景。
---
*注：以上内容基于学术文献常见模式虚构，如需真实文献请查阅PubMed、Web of Science等平台。*
**Background of Recombinant Human GRCC9 Protein**
Recombinant human GRCC9 (Gene Regulatory and Cell Cycle 9) is a engineered protein derived from the human GRCC9 gene, which encodes a regulatory factor involved in cell cycle progression and transcriptional modulation. The GRCC9 protein contains conserved functional domains, including a zinc finger motif and a leucine-rich repeat region, suggesting roles in DNA binding and protein-protein interactions. Studies have linked GRCC9 to the regulation of genes associated with cell proliferation, differentiation, and apoptosis, particularly in tissues such as the nervous system, liver, and hematopoietic cells.
Its recombinant form is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable high-purity, functional studies. Recombinant GRCC9 has become a critical tool for investigating molecular pathways in diseases like cancer, neurodegenerative disorders, and immune dysregulation. For example, aberrant GRCC9 expression has been observed in glioblastoma and leukemia, implicating its potential as a therapeutic target or biomarker.
Recent research also explores its interaction with signaling cascades, such as the Wnt/β-catenin and PI3K/AKT pathways, highlighting its cross-talk with oncogenic or neuroprotective mechanisms. Despite progress, the full scope of GRCC9’s biological functions and clinical relevance remains under investigation, driving demand for reliable recombinant variants to advance mechanistic and translational studies.
×
