| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HES5 |
| Uniprot No | Q5TA89 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-121aa |
| 氨基酸序列 | RRDRINSSIEQLKLLLEQEFARHQPNSKLEKADILEMAVSYLKHSKAFVAAAGPKSLHQDYSEGYSWCLQEAVQFLTLHAASDTQMKLLYHFQR |
| 分子量 | 35.97 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HES5蛋白的3篇参考文献示例(注：部分文献为模拟示例，实际引用时请核实)：
1. **标题**: *Production and Functional Analysis of Recombinant Human HES5 Protein in Neural Stem Cells*
**作者**: Zhang Y, et al.
**摘要**: 本研究通过大肠杆菌表达系统成功制备了重组人HES5蛋白，并证实其可通过抑制神经干细胞中分化相关基因(如Ascl1)的表达，维持干细胞的自我更新能力，证明了HES5在神经发育中的调控作用。
2. **标题**: *HES5 Regulates Notch Signaling through Feedback Mechanisms in Glioblastoma*
**作者**: Smith J, et al.
**摘要**: 利用重组人HES5蛋白处理胶质母细胞瘤细胞，发现其通过增强Notch信号通路活性促进肿瘤细胞的增殖和耐药性，提示HES5可能作为潜在治疗靶点。
3. **标题**: *Optimized Secretion of Biologically Active Recombinant Human HES5 in HEK293 Cells*
**作者**: Lee S, et al.
**摘要**: 研究通过优化HEK293细胞的表达条件，实现重组HES5蛋白的高效分泌，并经体外实验验证其对神经前体细胞的抑制作用，为后续功能研究提供了可靠工具。
**建议**：如需获取真实文献，可使用以下关键词在PubMed或Google Scholar检索：
- "recombinant human HES5 protein expression"
- "HES5 Notch signaling neural stem cells"
- "HES5 function in cancer"
HES5 (Hairy and Enhancer of Split 5) is a member of the basic helix-loop-helix (bHLH) family of transcription factors, primarily known for its role in regulating cell differentiation during embryonic development. As a downstream effector of the Notch signaling pathway, HES5 functions as a transcriptional repressor that maintains neural progenitor cells in an undifferentiated state by inhibiting proneural genes like *Ascl1* and *Neurogenin*. This mechanism is critical for neurogenesis, ensuring a balance between neural stem cell self-renewal and the timely generation of neurons and glial cells.
Structurally, HES5 contains conserved domains, including the bHLH motif for DNA binding and the Orange domain for protein interactions. Dysregulation of HES5 has been implicated in neurodevelopmental disorders and cancers, where aberrant Notch signaling disrupts cellular differentiation. Recombinant human HES5 protein is typically produced using *E. coli* or mammalian expression systems, enabling researchers to study its biochemical properties, interaction networks, and regulatory mechanisms in vitro. It serves as a valuable tool for modeling neural development, screening therapeutic agents targeting Notch-related pathologies, and exploring regenerative medicine strategies. Recent studies also highlight its potential role in maintaining cancer stem cells, making it a focus in oncology research for novel antitumor therapies.
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