| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HKDC1 |
| Uniprot No | Q2TB90 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-917aa |
| 氨基酸序列 | MFAVHLMAFYFSKLKEDQIKKVDRFLYHMRLSDDTLLDIMRRFRAEMEKGLAKDTNPTAAVKMLPTFVRAIPDGSENGEFLSLDLGGSKFRVLKVQVAEEGKRHVQMESQFYPTPNEIIRGNGTELFEYVADCLADFMKTKDLKHKKLPLGLTFSFPCRQTKLEEGVLLSWTKKFKARGVQDTDVVSRLTKAMRRHKDMDVDILALVNDTVGTMMTCAYDDPYCEVGVIIGTGTNACYMEDMSNIDLVEGDEGRMCINTEWGAFGDDGALEDIRTEFDRELDLGSLNPGKQLFEKMISGLYLGELVRLILLKMAKAGLLFGGEKSSALHTKGKIETRHVAAMEKYKEGLANTREILVDLGLEPSEADCIAVQHVCTIVSFRSANLCAAALAAILTRLRENKKVERLRTTVGMDGTLYKIHPQYPKRLHKVVRKLVPSCDVRFLLSESGSTKGAAMVTAVASRVQAQRKQIDRVLALFQLTREQLVDVQAKMRAELEYGLKKKSHGLATVRMLPTYVCGLPDGTEKGKFLALDLGGTNFRVLLVKIRSGRRSVRMYNKIFAIPLEIMQGTGEELFDHIVQCIADFLDYMGLKGASLPLGFTFSFPCRQMSIDKGTLIGWTKGFKATDCEGEDVVDMLREAIKRRNEFDLDIVAVVNDTVGTMMTCGYEDPNCEIGLIAGTGSNMCYMEDMRNIEMVEGGEGKMCINTEWGGFGDNGCIDDIWTRYDTEVDEGSLNPGKQRYEKMTSGMYLGEIVRQILIDLTKQGLLFRGQISERLRTRGIFETKFLSQIESDRLALLQVRRILQQLGLDSTCEDSIVVKEVCGAVSRRAAQLCGAGLAAIVEKRREDQGLEHLRITVGVDGTLYKLHPHFSRILQETVKELAPRCDVTFMLSEDGSGKGAALITAVAKRLQQAQKEN |
| 分子量 | 102 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人HKDC1蛋白的示例参考文献(内容为模拟，建议进一步查阅真实数据库验证)：
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1. **文献名称**：*"Expression and Purification of Recombinant Human HKDC1 Protein for Functional Studies"*
**作者**：Zhang Y, et al.
**摘要**：研究报道了利用杆状病毒-昆虫细胞系统高效表达重组人HKDC1蛋白，并通过亲和层析纯化获得高纯度蛋白，证实其具有结合葡萄糖及线粒体外膜的能力，为后续功能研究提供了工具。
2. **文献名称**：*"HKDC1 Interacts with Mitochondrial VDAC1 to Modulate Cellular Glucose Metabolism"*
**作者**：Chen L, et al.
**摘要**：通过重组人HKDC1蛋白的体外实验，揭示了其与电压依赖性阴离子通道(VDAC1)的相互作用，表明HKDC1在调控线粒体糖摄取和ATP生成中的关键作用，可能影响癌症代谢重编程。
3. **文献名称**：*"Crystal Structure Analysis of Recombinant HKDC1 Reveals Unique Hexokinase Activity"*
**作者**：Tanaka K, et al.
**摘要**：本研究解析了重组人HKDC1蛋白的晶体结构，发现其底物结合位点与传统己糖激酶不同，并证实其对五碳糖的高亲和力，提示HKDC1在特定病理生理条件下的独特代谢功能。
4. **文献名称**：*"Role of HKDC1 in Hepatic Lipid Metabolism: Insights from Recombinant Protein Overexpression"*
**作者**：Wang X, et al.
**摘要**：利用重组HKDC1蛋白过表达模型，发现HKDC1通过影响线粒体-内质网接触位点(MAMs)调控肝脏脂质稳态，其缺失可能导致非酒精性脂肪肝的发生。
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**注意**：以上文献信息为示例，实际研究需通过PubMed(https://pubmed.ncbi.nlm.nih.gov)或Google Scholar等平台检索关键词“recombinant HKDC1”“human HKDC1 protein”等获取准确结果。
**Background of Recombinant Human HKDC1 Protein**
Recombinant human HKDomain-containing protein 1 (HKDC1) is a member of the hexokinase family, involved in glucose metabolism and mitochondrial function. Initially identified as a paralog of hexokinase 2 (HK2), HKDC1 contains two conserved hexokinase domains but exhibits unique regulatory and functional properties. It localizes to mitochondria, where it interacts with the voltage-dependent anion channel (VDAC) to facilitate glucose phosphorylation and maintain mitochondrial stability.
HKDC1 has garnered attention for its dual roles in metabolic homeostasis and disease. Studies link it to glycolysis, mitochondrial quality control, and mitophagy, influencing cellular energy production and stress responses. Its expression is upregulated in metabolic disorders (e.g., diabetes, non-alcoholic fatty liver disease) and certain cancers, suggesting involvement in pathological glucose utilization and tumor progression.
Research using recombinant HKDC1 has clarified its structure-function relationships, post-translational modifications, and interactions with metabolic signaling pathways (e.g., mTOR, AMPK). Additionally, HKDC1 is explored as a potential biomarker or therapeutic target, particularly in cancers reliant on aerobic glycolysis. Recombinant versions, produced via bacterial or mammalian systems, enable mechanistic studies and drug screening, advancing our understanding of its physiological and pathological significance. Further investigation is needed to unravel its tissue-specific roles and therapeutic potential.
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