| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAD50 |
| Uniprot No | Q92878 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-235aa |
| 氨基酸序列 | MSRIEKMSILGVRSFGIEDKDKQIITFFSPLTILVGPNGAGKTTIIECLKYICTGDFPPGTKGNTFVHDPKVAQETDVRAQIRLQFRDVNGELIAVQRSMVCTQKSKKTEFKTLEGVITRTKHGEKVSLSSKCAEIDREMISSLGVSKAVLNNVIFCHQEDSNWPLSEGKALKQKFDEIFSATRYIKALETLRQVRQTQGQKVKEYQMELKYLKQYKEKACEIRDQITSKEAQLT |
| 预测分子量 | 34.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAD50重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"The Rad50 zinc-hook is a structure joining Mre11 complexes in DNA recombination and repair"*
**作者**: Hopfner, K.P., et al.
**摘要**: 通过X射线晶体学解析了RAD50蛋白的结构，揭示了其锌钩结构域介导Mre11复合体二聚化的机制，表明其在DNA损伤修复中通过桥接断裂DNA末端发挥作用。
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2. **文献名称**: *"The Mre11 complex and the metabolism of chromosome breaks: the importance of communicating and holding things together"*
**作者**: Stracker, T.H., Williams, B.R.
**摘要**: 综述了Mre11-RAD50-Nbs1(MRN)复合体的功能，重点强调RAD50的ATP酶活性在DNA末端识别、加工及同源重组修复中的核心作用。
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3. **文献名称**: *"Human Rad50 is physically associated with human Mre11: Identification of a conserved multiprotein complex implicated in recombination/repair"*
**作者**: Dolganov, G.M., et al.
**摘要**: 首次证明人源RAD50与MRE11形成稳定复合物，并发现其在电离辐射诱导的DNA双链断裂修复中协同调控细胞周期检查点及重组修复。
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(注：以上文献信息基于领域内经典研究，若需具体发表年份或期刊，建议通过PubMed等数据库进一步核实。)
The RAD50 recombination protein is a critical component of the DNA damage response machinery, playing a central role in maintaining genomic stability. It belongs to the structural maintenance of chromosomes (SMC) protein family, characterized by conserved ATPase domains and coiled-coil regions. RAD50 forms a conserved complex with MRE11 and NBS1 (Xrs2 in yeast), collectively known as the MRN/MRX complex, which is essential for detecting, processing, and signaling DNA double-strand breaks (DSBs) – one of the most lethal types of DNA damage.
Functionally, RAD50 acts as a molecular bridge that coordinates DNA ends through its flexible coiled-coil domains. Its ATPase activity facilitates conformational changes required for DNA binding, end-tethering, and resection. The protein contributes to multiple DSB repair pathways, including homologous recombination (HR) and non-homologous end joining (NHEJ). Additionally, RAD50 participates in checkpoint activation by recruiting signaling kinases like ATM/ATR to damage sites. Beyond repair, it supports telomere maintenance and meiotic recombination.
Recombinant RAD50 proteins, typically expressed in bacterial or insect cell systems, retain these biochemical properties and are widely used in vitro to study DNA repair mechanisms. Researchers employ purified RAD50 to investigate its ATP-dependent DNA unwinding, nuclease regulation through MRE11 interaction, and structural dynamics during DNA end recognition. Mutations in RAD50 are associated with hereditary cancer predisposition syndromes and chromosomal instability disorders, making its recombinant form valuable for functional studies of disease-associated variants. Current therapeutic strategies targeting the MRN complex in cancer therapy further highlight its biomedical relevance.
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