| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DKK 4 |
| Uniprot No | Q9UBT3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-224aa |
| 氨基酸序列 | LVLDFNNIRSSADLHGARKGSQCLSDTDCNTRKFCLQPRDEKPFCATCRGLRRRCQRDAMCCPGTLCVNDVCTTMEDATPILERQLDEQDGTHAEGTTGHPVQENQPKRKPSIKKSQGRKGQEGESCLRTFDCGPGLCCARHFWTKICKPVLLEGQVCSRRGHKDTAQAPEIFQRCDCGPGLLCRSQLTSNRQHARLRVCQKIEKL |
| 预测分子量 | 27.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **DKK4重组蛋白** 的3条参考文献示例(内容基于领域内常见研究方向构造，供参考)：
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1. **文献名称**：*"Recombinant DKK4 Protein Inhibits Wnt/β-Catenin Signaling in Colorectal Cancer Cells"*
**作者**：Chen et al.
**摘要**：研究通过表达并纯化人源DKK4重组蛋白，发现其能够特异性结合LRP5/6受体，抑制Wnt/β-catenin信号通路，从而抑制结直肠癌细胞增殖和迁移，提示DKK4可能作为潜在抗癌靶点。
2. **文献名称**：*"Expression and Functional Characterization of DKK4 Recombinant Protein in Stem Cell Maintenance"*
**作者**：Wang et al.
**摘要**：利用哺乳动物细胞系统成功表达具有生物活性的DKK4重组蛋白，并证明其通过拮抗Wnt信号调控肠道干细胞的自我更新，为组织再生研究提供工具。
3. **文献名称**：*"Structural Analysis of Recombinant DKK4 Reveals Its Role in Tumor Microenvironment Remodeling"*
**作者**：Kimura et al.
**摘要**：通过冷冻电镜解析DKK4重组蛋白的结构，阐明其与微环境基质蛋白的相互作用机制，发现DKK4可通过调节基质硬度促进肿瘤耐药性，为靶向治疗提供依据。
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如需真实文献，建议在 **PubMed** 或 **Google Scholar** 中检索关键词“DKK4 recombinant protein”或“DKK4 Wnt signaling”。
**Background of DKK4 Recombinant Protein**
DKK4 (Dickkopf-related protein 4) is a member of the Dickkopf family of secreted glycoproteins, known for their role in modulating Wnt signaling pathways. The Wnt pathway is critical for embryonic development, tissue homeostasis, and cell proliferation, and its dysregulation is linked to cancers, bone diseases, and developmental disorders. DKK proteins typically act as antagonists of Wnt signaling by binding to low-density lipoprotein receptor-related proteins 5/6 (LRP5/6), co-receptors essential for canonical Wnt signal transduction. However, DKK4 exhibits context-dependent activity, functioning as either a Wnt inhibitor or activator depending on cellular conditions and receptor interactions.
DKK4 is expressed in specific tissues during embryogenesis, including the developing limbs, kidneys, and nervous system, suggesting roles in organogenesis. In adults, abnormal DKK4 expression is associated with cancers (e.g., colorectal, breast, and liver cancers), where it may promote tumor growth, metastasis, or drug resistance by disrupting Wnt-regulated processes. Its dual regulatory effects on Wnt signaling complicate its functional characterization, necessitating further research to clarify its mechanisms.
Recombinant DKK4 protein is produced using genetic engineering techniques, often in mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications. Purified via affinity chromatography, the protein retains bioactivity for experimental applications, such as studying Wnt pathway dynamics, screening therapeutic agents, or exploring DKK4's role in disease models.
Current research focuses on DKK4's potential as a diagnostic biomarker or therapeutic target, particularly in cancers with aberrant Wnt signaling. Challenges include understanding its tissue-specific roles and resolving conflicting data on its pro- or anti-tumor effects. Recombinant DKK4 serves as a vital tool in these investigations, bridging molecular insights to potential clinical applications.
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艾普蒂生物自主研发并建立综合性重组蛋白生产和抗体开发技术平台，包括： 哺乳动物细胞表达平台：利用哺乳动物细胞精准修饰蛋白，产出与天然蛋白相似的重组蛋白，用于药物研发、细胞治疗等。 杂交瘤开发平台：通过细胞融合筛选出稳定分泌单克隆抗体的杂交瘤细胞株，优化后的技术让抗体亲和力与特异性更高，应用于疾病诊断、免疫治疗等领域。 单 B 细胞筛选平台：FACS 用荧光标记和流式细胞仪快速分选特定 B 细胞；Beacon® 基于微流控技术，单细胞水平捕获、分析 B 细胞，挖掘抗体多样性，缩短开发周期。 凭借这些平台，艾普蒂生物为客户提供优质试剂和专业 CRO 技术服务，推动生物科技发展。
艾普蒂生物在重组蛋白和天然蛋白开发领域经验十分丰富，拥有超过 2 万种重组蛋白的开发案例。在四大重组蛋白表达平台的运用上，艾普蒂生物不仅经验老到，还积累了详实的成功案例。针对客户的工业化生产需求，我们能够定制并优化实验方案。通过小试探索、工艺放大以及条件优化等环节，对重组蛋白基因序列进行优化，全面探索多种条件，精准找出最契合客户需求的生产方法。 此外，公司还配备了自有下游验证平台，可对重组蛋白展开系统的质量检测与性能测试，涵盖蛋白互作检测、活性验证、内毒素验证等，全方位保障产品质量。 卡梅德生物同样重视蛋白工艺开发，确保生产出的蛋白质具备所需的纯度、稳定性与生物活性，这对于保障药物的安全性和有效性起着关键作用 ，与艾普蒂生物共同推动着行业的发展。
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