| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX5 |
| Uniprot No | P17844 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-614aa |
| 氨基酸序列 | MSGYSSDRDRGRDRGFGAPRFGGSRAGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPANVMDVIARQNFTEPTAIQAQGWPVALSGLDMVGVAQTGSGKTLSYLLPAIVHINHQPFLERGDGPICLVLAPTRELAQQVQQVAAEYCRACRLKSTCIYGGAPKGPQIRDLERGVEICIATPGRLIDFLECGKTNLRRTTYLVLDEADRMLDMGFEPQIRKIVDQIRPDRQTLMWSATWPKEVRQLAEDFLKDYIHINIGALELSANHNILQIVDVCHDVEKDEKLIRLMEEIMSEKENKTIVFVETKRRCDELTRKMRRDGWPAMGIHGDKSQQERDWVLNEFKHGKAPILIATDVASRGLDVEDVKFVINYDYPNSSEDYIHRIGRTARSTKTGTAYTFFTPNNIKQVSDLISVLREANQAINPKLLQLVEDRGSGRSRGRGGMKDDRRDRYSAGKRGGFNTFRDRENYDRGYSSLLKRDFGAKTQNGVYSAANYTNGSFGSNFVSAGIQTSFRTGNPTGTYQNGYDSTQQYGSNVPNMHNGMNQQAYAYPATAAAPMIGYPMPTGYSQ |
| 预测分子量 | 74.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于DDX5重组蛋白的参考文献信息(注：文献为虚拟示例，仅用于展示格式)：
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1. **标题**: *Recombinant DDX5 enhances viral RNA unwinding in hepatitis C replication*
**作者**: Smith J, et al.
**摘要**: 研究利用大肠杆菌表达系统纯化重组人DDX5蛋白，证明其在体外通过ATP依赖的解旋酶活性促进HCV RNA二级结构的解链，揭示了DDX5在丙肝病毒复制中的分子机制。
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2. **标题**: *Structural analysis of recombinant human DDX5 in transcriptional regulation*
**作者**: Lee H, et al.
**摘要**: 通过冷冻电镜解析了重组DDX5与β-catenin蛋白复合物的三维结构，揭示了DDX5通过结合Wnt信号通路关键蛋白调控结直肠癌基因转录的分子基础。
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3. **标题**: *DDX5 recombinant protein as a potential biomarker in breast cancer*
**作者**: Wang Y, et al.
**摘要**: 研究发现高表达的重组DDX5蛋白通过激活ERα的转录活性促进乳腺癌细胞增殖，提出其可作为乳腺癌预后标志物及治疗靶点。
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**说明**：以上文献为模拟内容，实际研究中建议通过PubMed或Web of Science检索关键词“recombinant DDX5 protein”获取真实文献。
**Background of DDX5 Recombinant Protein**
DDX5. also known as p68 or DEAD-box helicase 5. is a conserved RNA helicase belonging to the DEAD-box protein family. These proteins are characterized by the presence of a DEAD (Asp-Glu-Ala-Asp) motif, which is critical for ATP-dependent RNA unwinding and remodeling activities. DDX5 plays multifaceted roles in RNA metabolism, including transcription, splicing, ribosome biogenesis, and RNA decay, by modulating RNA secondary structures and RNA-protein interactions. It is implicated in cellular processes such as cell cycle regulation, differentiation, and stress responses. Dysregulation of DDX5 has been linked to cancers, viral infections, and neurodegenerative diseases, underscoring its biological and clinical significance.
Recombinant DDX5 protein is engineered through molecular cloning, typically expressed in *E. coli* or mammalian cell systems, followed by purification using affinity tags (e.g., His-tag). This purified protein retains enzymatic activity, enabling functional studies *in vitro*. Researchers utilize recombinant DDX5 to investigate its helicase activity, ATPase kinetics, and interactions with nucleic acids or partner proteins (e.g., p72. β-catenin, or viral proteins). Structural studies, including crystallography and cryo-EM, have mapped its core helicase domains and flexible termini, which mediate substrate specificity and regulatory post-translational modifications (e.g., phosphorylation, acetylation).
The protein’s role in cancer—particularly in promoting oncogene expression and stabilizing cancer-related mRNAs—has driven interest in DDX5 as a therapeutic target. Additionally, its involvement in viral replication (e.g., HIV, hepatitis C) highlights its utility in virology research. Recombinant DDX5 also serves as a tool for *in vitro* RNA manipulation, aiding in studies of RNA structure-function relationships. Overall, DDX5 recombinant protein is a vital resource for dissecting helicase mechanisms and exploring therapeutic strategies in disease contexts.
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