| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GATA1 |
| Uniprot No | P15976 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-413aa |
| 氨基酸序列 | MASMTGGQQMGRGHHHHHHENLYFQGEFPGLGSLGTSEPLPQFVDPALVS STPESGVFFPSGPEGLDAAASSTAPSTATAAAAALAYYRDAEAYRHSPVF QVYPLLNCMEGIPGGSPYAGWAYGKTGLYPASTVCPTREDSPPQAVEDLD GKGSTSFLETLKTERLSPDLLTLGPALPSSLPVPNSAYGGPDFSSTFFSP TGSPLNSAAYSSPKLRGTLPLPPCEARECVNCGATATPLWRRDRTGHYLC NACGLYHKMNGQNRPLIRPKKRLIVSKRAGTQCTNCQTTTTTLWRRNASG DPVCNACGLYYKLHQVNRPLTMRKDGIQTRNRKASGKGKKKRGSSLGGTG AAEGPAGGFMVVAGGSGSGNCGEVASGLTLGPPGTAHLYQGLGPVVLSGP VSHLMPFPGPLLGSPTGSFPTGPMPPTTSTTVVAPLSS |
| 预测分子量 | 46 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与GATA1重组蛋白相关的代表性文献(内容基于模拟文献概括，供参考)：
---
1. **文献名称**：*Recombinant GATA1 Protein Production in E. coli and Functional Analysis of DNA Binding*
**作者**：Shimizu R, Yamamoto M
**摘要**：该研究报道了利用大肠杆菌表达系统高效表达并纯化重组人源GATA1蛋白，验证其与靶DNA序列的结合活性，并揭示其锌指结构域的关键作用。
2. **文献名称**：*Structural Characterization of Recombinant Murine GATA1 and Its Role in Erythropoiesis*
**作者**：Trainor CD, Omichinski JG
**摘要**：通过昆虫细胞表达系统获得高纯度小鼠GATA1重组蛋白，结合X射线晶体学解析其结构，证明其C端结构域对红系祖细胞分化的调控机制。
3. **文献名称**：*Dysregulated GATA1 Splicing Variants in Myelodysplastic Syndromes: Recombinant Protein Rescue Assays*
**作者**：Crispino JD, Weiss MJ
**摘要**：研究构建了GATA1剪接突变体的重组蛋白，通过体外细胞实验证实特定突变导致DNA结合能力丧失，并与骨髓增生异常综合征的发病相关。
---
注：以上文献为示例性质，实际引用时请以真实数据库(如PubMed、Web of Science)检索结果为准。
**Background of GATA1 Recombinant Protein**
GATA1 is a critical transcription factor belonging to the GATA family, which recognizes and binds to the consensus DNA motif (A/T)GATA(A/G). It plays a central role in hematopoiesis, particularly in the development and differentiation of erythroid cells, megakaryocytes, mast cells, and eosinophils. GATA1 regulates the expression of genes involved in hemoglobin synthesis, cell cycle control, and apoptosis, ensuring proper maturation of red blood cells and platelet-producing megakaryocytes. Mutations or dysregulation of GATA1 are linked to hematopoietic disorders, including Diamond-Blackfan anemia, myelodysplastic syndromes, and acute megakaryoblastic leukemia.
Recombinant GATA1 protein is produced using *in vitro* expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and functionality. It typically retains conserved structural features, such as two zinc finger domains essential for DNA binding and protein interactions. The N-terminal zinc finger stabilizes DNA binding, while the C-terminal finger mediates interactions with co-factors like FOG1. Recombinant GATA1 is widely used to study gene regulatory mechanisms, model diseases *in vitro*, and screen therapeutic compounds targeting hematologic malignancies.
In research, it serves as a tool for chromatin immunoprecipitation (ChIP), electrophoretic mobility shift assays (EMSAs), and transcriptional activation studies. Its applications extend to stem cell engineering, where GATA1 overexpression or inhibition helps unravel pathways driving hematopoietic lineage commitment. Overall, GATA1 recombinant protein bridges molecular insights into blood cell development and translational efforts for treating hematologic disorders.
×
