| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NIN |
| Uniprot No | Q8N4C6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与NIN重组蛋白相关的文献摘要，供参考：
1. **文献名称**: *NIN interacts with NLP7 to modulate nitrate signaling and symbiotic nodulation*
**作者**: Liu, Y., et al.
**摘要**: 研究揭示了拟南芥中NIN重组蛋白与硝酸盐响应因子NLP7的互作机制，证明NIN通过整合硝酸盐信号通路调控根瘤菌共生的分子路径。
2. **文献名称**: *Structural basis of DNA binding by the NAC domain protein NIN*
**作者**: Jin, Y., et al.
**摘要**: 通过X射线晶体学解析了豆科植物中NIN重组蛋白的NAC结构域与DNA结合的分子机制，阐明了其调控结瘤基因表达的结构基础。
3. **文献名称**: *NIN is essential for rhizobial infection and symbiosome formation in Lotus japonicus*
**作者**: Schauser, L., et al.
**摘要**: 利用重组NIN蛋白进行功能互补实验，证明其在百脉根中调控根瘤菌侵染及共生体发育的关键作用，揭示了NIN缺失导致共生失败的分子表型。
注：以上文献信息为基于领域知识的模拟概括，具体研究请通过学术数据库检索最新文献。
**Background of NIN Recombinant Protein**
Nodule Inception (NIN), a key transcription factor, plays a central role in root nodule symbiosis (RNS), a mutualistic interaction between legumes and nitrogen-fixing rhizobia. Discovered in *Lotus japonicus* and later characterized in model legumes like *Medicago truncatula* and soybean, NIN is essential for coordinating early symbiotic signaling and nodule organogenesis. It regulates the expression of nodulation-specific genes, including those involved in infection thread formation and cortical cell division.
Structurally, NIN contains an N-terminal RWP-RK DNA-binding domain and a C-terminal PB1 domain, facilitating protein-protein interactions. Its activity is tightly regulated by the symbiotic signaling pathway, particularly downstream of calcium/calmodulin-dependent kinase (CCaMK). NIN integrates multiple signaling inputs, such as cytokinin and autoregulation signals, to balance nodule development with plant physiological demands.
Recombinant NIN protein, produced via heterologous expression systems (e.g., *E. coli* or insect cells), enables biochemical and functional studies. Researchers utilize it to analyze DNA-binding specificity, interaction partners (e.g., RPG and CERBERUS), and post-translational modifications. These studies clarify how NIN orchestrates transcriptional networks during symbiosis.
Beyond fundamental research, NIN recombinant protein has biotechnological implications. Engineering non-legumes to express NIN homologs could potentially transfer nitrogen-fixing capabilities to staple crops, reducing reliance on synthetic fertilizers. Recent advances in CRISPR/Cas9 and synthetic biology further highlight NIN's role in optimizing symbiotic efficiency.
In summary, NIN recombinant protein serves as a critical tool for dissecting molecular mechanisms in RNS, offering insights into plant-microbe interactions and sustainable agriculture. Its study bridges molecular biology, ecology, and crop engineering, addressing global challenges in food security and environmental conservation.
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