| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MTRF1L |
| Uniprot No | Q9UGC7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-380 aa |
| 活性数据 | SSGS PPLEELFTRG GPLRTFLERQ AGSEAHLKVR RPELLAVIKL LNEKERELRE TEHLLHDENE DLRKLAENEI TLCQKEITQL KHQIILLLVP SEETDENDLI LEVTAGVGGQ EAMLFTSEIF DMYQQYAAFK RWHFETLEYF PSELGGLRHA SASIGGSEAY RHMKFEGGVH RVQRVPKTEK QGRVHTSTMT VAILPQPTEI NLVINPKDLR IDTKRASGAG GQHVNTTDSA VRIVHLPTGV VSECQQERSQ LKNKELAMTK LRAKLYSMHL EEEINKRQNA RKIQIGSKGR SEKIRTYNFP QNRVTDHRIN KTLHDLETFM QGDYLLDELV QSLKEYADYE SLVEIISQKV |
| 分子量 | 43.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人MTRF1L蛋白相关的参考文献示例(部分内容为模拟示例，实际文献需通过学术数据库验证)：
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1. **"Structural Insights into the Mitochondrial Translation Release Factor MTRF1L and Its Role in Termination"**
*Zhang et al., 2018*
摘要：本研究通过大肠杆菌重组表达了人源MTRF1L蛋白，解析其晶体结构，揭示了其与线粒体核糖体结合的机制，并验证其在翻译终止过程中促进多肽链释放的功能，与细菌释放因子RF1具有功能保守性。
2. **"MTRF1L Knockdown Disrupts Mitochondrial Protein Synthesis and Induces Apoptosis in Cancer Cells"**
*Lee et al., 2020*
摘要：利用重组MTRF1L蛋白进行体外功能实验，发现其敲低导致线粒体翻译缺陷，引发癌细胞氧化应激和凋亡，提示其在肿瘤代谢调控中的潜在作用。
3. **"Recombinant MTRF1L Expression and Functional Characterization in Neurodegenerative Disease Models"**
*Singh & Smith, 2021*
摘要：通过哺乳动物细胞系统表达重组MTRF1L蛋白，发现其在帕金森病模型中能部分恢复线粒体翻译效率，为神经退行性疾病治疗提供新靶点。
4. **"Functional Redundancy between MTRF1 and MTRF1L in Vertebrate Mitochondrial Translation"**
*Garcia et al., 2019*
摘要：比较重组MTRF1和MTRF1L蛋白的功能差异，发现两者在线粒体翻译终止中存在部分冗余，但在组织特异性表达中发挥不同作用。
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**说明**：上述文献为示例，实际研究中需通过PubMed、Web of Science等平台以 **“MTRF1L”** 或 **“mitochondrial translation release factor 1-like”** 等关键词检索真实文献。MTRF1L研究相对前沿，建议结合最新数据库查询具体成果。
**Background of Recombinant Human MTRF1L Protein**
MTRF1L (Mitochondrial Translational Release Factor 1-Like) is a protein involved in mitochondrial translation termination, sharing homology with bacterial release factor RF1. It is critical for terminating protein synthesis within mitochondria by recognizing stop codons during mRNA translation, facilitating the release of nascent polypeptides from mitochondrial ribosomes. As mitochondria rely on their own translational machinery to produce components of the electron transport chain (ETC), MTRF1L plays a vital role in maintaining mitochondrial function and cellular energy homeostasis.
Dysregulation of MTRF1L has been implicated in mitochondrial disorders and metabolic diseases, as impaired translation termination disrupts ETC complex assembly, leading to oxidative phosphorylation defects. Studies suggest its potential involvement in apoptosis and cellular stress responses, linking it to neurodegenerative diseases, cancer, and aging. Recombinant human MTRF1L protein, typically expressed in *E. coli* or mammalian systems, is utilized to study its structural and functional properties, decipher molecular mechanisms in mitochondrial diseases, and screen therapeutic agents targeting mitochondrial dysfunction.
Current research focuses on elucidating its interaction partners, regulatory pathways, and structural insights to advance understanding of mitochondrial biology and develop precision therapies.
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