| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MTRF1 |
| Uniprot No | O75570 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-151 aa |
| 活性数据 | MNRHLCVWLFRHPSLNGYLQCHIQLHSHQFRQIHLDTRLQVFRQNRNCILHLLSKNWSRRYCHQDTKMLWKHKALQKYMENLSKEYQTLEQCLQHIPVNEENRRSLNRRHAELAPLAAIYQEIQETEQAIEELESMCKKTESCSVAQAGMQ |
| 分子量 | 44.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MTRF1蛋白的3篇代表性参考文献(示例为虚构内容，仅供参考格式)：
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1. **文献名称**: *"Functional characterization of recombinant human mitochondrial translation release factor 1 (mtRF1) in termination of mitochondrial translation"*
**作者**: Lee S, et al.
**摘要**: 研究重组人mtRF1蛋白在体外线粒体翻译系统中的功能，证实其通过识别终止密码子UAA/UAG调控翻译终止，并依赖ATP水解发挥作用。
2. **文献名称**: *"Crystal structure of human MTRF1 reveals insights into mitochondrial translation machinery"*
**作者**: Zhang Y, et al.
**摘要**: 报道重组人MTRF1蛋白的晶体结构，揭示其与线粒体核糖体及tRNA的相互作用机制，为理解线粒体疾病相关突变提供结构基础。
3. **文献名称**: *"Impaired MTRF1 activity disrupts mitochondrial protein synthesis and exacerbates oxidative stress in cardiomyopathy"*
**作者**: Gupta R, et al.
**摘要**: 通过重组MTRF1蛋白回补实验，证明其功能缺陷导致心肌细胞线粒体翻译障碍，进而引发氧化应激和心衰表型。
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注：若需真实文献，请提供更具体的MTRF1研究方向或通过PubMed/Google Scholar检索PMID编号进行精确定位。
Recombinant human mitochondrial translation release factor 1 (MTRF1), also known as mtRF1. is a nuclear-encoded protein critical for mitochondrial protein synthesis. Mitochondria, often termed the "powerhouses" of eukaryotic cells, possess their own circular DNA (mtDNA) encoding essential components of the oxidative phosphorylation (OXPHOS) system. Unlike cytoplasmic translation, mitochondrial translation relies on specialized machinery, including factors like MTRF1. which assists in terminating polypeptide chain elongation. Specifically, MTRF1 recognizes stop codons (UAA/UAG) on mitochondrial mRNAs, facilitating the release of nascent polypeptides from mitochondrial ribosomes. Dysfunction in mitochondrial translation is linked to severe metabolic disorders, neurodegenerative diseases, and aging-related conditions.
Recombinant MTRF1 is produced using biotechnological platforms (e.g., bacterial or mammalian expression systems) to study its structural and functional roles. Its recombinant form enables precise investigations into mitochondrial translation mechanisms, protein-mRNA interactions, and mutations associated with diseases. Research leveraging recombinant MTRF1 has advanced understanding of mitochondrial gene expression regulation and potential therapeutic strategies targeting OXPHOS deficiencies. Additionally, it serves as a tool for developing diagnostics for mitochondrial disorders. The recombinant protein’s high purity and scalability make it indispensable for both basic research and translational applications, bridging gaps in mitochondrial biology and medicine.
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