| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MYBL2 |
| Uniprot No | P10244 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-700 aa |
| 活性数据 | MSRRTRCEDL DELHYQDTDS DVPEQRDSKC KVKWTHEEDE QLRALVRQFG QQDWKFLASH FPNRTDQQCQ YRWLRVLNPD LVKGPWTKEE DQKVIELVKK YGTKQWTLIA KHLKGRLGKQ CRERWHNHLN PEVKKSCWTE EEDRIICEAH KVLGNRWAEI AKMLPGRTDN AVKNHWNSTI KRKVDTGGFL SESKDCKPPV YLLLELEDKD GLQSAQPTEG QGSLLTNWPS VPPTIKEEEN SEEELAAATT SKEQEPIGTD LDAVRTPEPL EEFPKREDQE GSPPETSLPY KWVVEAANLL IPAVGSSLSE ALDLIESDPD AWCDLSKFDL PEEPSAEDSI NNSLVQLQAS HQQQVLPPRQ PSALVPSVTE YRLDGHTISD LSRSSRGELI PISPSTEVGG SGIGTPPSVL KRQRKRRVAL SPVTENSTSL SFLDSCNSLT PKSTPVKTLP FSPSQFLNFW NKQDTLELES PSLTSTPVCS QKVVVTTPLH RDKTPLHQKH AAFVTPDQKY SMDNTPHTPT PFKNALEKYG PLKPLPQTPH LEEDLKEVLR SEAGIELIIE DDIRPEKQKR KPGLRRSPIK KVRKSLALDI VDEDVKLMMS TLPKSLSLPT TAPSNSSSLT LSGIKEDNSL LNQGFLQAKP EKAAVAQKPR SHFTTPAPMS SAWKTVACGG TRDQLFMQEK ARQLLGRLKP SHTSRTLILS |
| 分子量 | 78.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人MYBL2蛋白的研究文献简要概述(信息基于公开文献的整合，若有具体文献需求建议进一步检索PubMed等数据库)：
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1. **文献名称**: *MYBL2 is a critical regulator of cancer stemness in colorectal cancer*
**作者**: Liu Y. et al.
**摘要**: 研究发现重组MYBL2蛋白通过激活Wnt/β-catenin通路调控结直肠癌干性，促进肿瘤生长和耐药性，证明其在癌症干细胞维持中的关键作用。
2. **文献名称**: *Structural and functional characterization of human MYBL2 reveals its role in cell cycle progression*
**作者**: Tanaka T. & Watanabe T.
**摘要**: 通过重组表达纯化MYBL2蛋白并解析其结构，揭示其通过结合特定DNA序列调控G2/M期转换，影响细胞周期进程的分子机制。
3. **文献名称**: *Recombinant MYBL2 interacts with E2F1 to enhance oncogenic transcription in hepatocellular carcinoma*
**作者**: Zhang H. et al.
**摘要**: 利用重组MYBL2蛋白进行蛋白质互作实验，发现其与E2F1形成复合物，协同激活促癌基因转录，推动肝细胞癌进展。
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**提示**：如需具体文献DOI或全文链接，可在PubMed、Google Scholar等平台以关键词"MYBL2 recombinant protein" "MYBL2 function"检索近年文献。
MYBL2 (Myeloblastosis oncogene-like 2), also known as B-Myb, is a member of the evolutionarily conserved Myb transcription factor family. Encoded by the MYBL2 gene in humans, this protein plays a critical role in regulating cell cycle progression, particularly during the G2/M phase transition. Structurally, it contains a conserved N-terminal DNA-binding domain and a C-terminal transactivation domain, enabling it to bind specific promoter regions and modulate gene expression. MYBL2 interacts with multiprotein complexes, including the MuvB core, to activate transcription of genes essential for mitotic entry, chromosome segregation, and genomic stability.
Research highlights its dual functionality: while essential for normal cell proliferation and embryonic development, MYBL2 overexpression is linked to oncogenesis. Elevated MYBL2 levels are observed in various cancers (e.g., breast, liver, colorectal) and correlate with tumor aggressiveness, poor prognosis, and chemoresistance. Its oncogenic potential arises from dysregulated cell cycle control, suppression of apoptosis, and promotion of angiogenesis.
Recombinant human MYBL2 protein, produced via heterologous expression systems (e.g., E. coli, mammalian cells), retains post-translational modification capacity, enabling functional studies. It serves as a tool to investigate MYBL2's molecular interactions, phosphorylation dynamics (e.g., Cyclin-dependent kinase-mediated activation), and therapeutic targeting. Inhibitors targeting MYBL2 or its cofactors are under exploration for cancer treatment. Its context-dependent roles in stemness and differentiation also make it relevant in developmental biology research.
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