| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MYBPH |
| Uniprot No | Q13203 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-477 aa |
| 活性数据 | MMEKNTSEGP ACSPEETASE SAKVPTAEPP GEVAVSESTR EEQVPKPQAP APQAPTASTA TKPAPPSEDV PSAPLLLTLD DVSSSSVTVS WEPPERLGRL GLQGYVLELC REGASEWVPV SARPMMVTQQ TVRNLALGDK FLLRVSAVSS AGAGPPAMLD QPIHIRENIE APKIRVPRHL RQTYIRQVGE TVNLQIPFQG KPKPQATWTH NGHALDSQRV SMRTGDQDSI LFIRSAQRSD SGRYELTVRV EDLEAKAVID ILVIEKPGPP SSIRLLDVWG CNAALQWTPP QDTGNTELLG YMVQKADKKT GQWFTVLERY HPTTCTISDL IIGNSYSFRV FSENLCGLST SATVTKELAH IQKADIAAKP KGFIERDFSE APSFTQPLAD HTSTPGYSTQ LFCSVRASPK PKIIWMKNKM EIQGNPKYRA LSEQGVCTLE IRKPSPFDSG VYTCKAINVL GEASVDCRLE VKASAAH |
| 分子量 | 52.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于重组人MYBPH蛋白的核心文献及其关键信息概要：
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1. **《MYBPH modulates EGFR signaling in head and neck squamous cell carcinoma》**
Authors: Tanaka N, et al.
**摘要**: 研究发现MYBPH作为表皮生长因子受体(EGFR)信号通路的调控因子，在头颈部鳞状细胞癌中通过抑制ERK磷酸化影响肿瘤细胞的迁移与侵袭能力，并成功构建重组人MYBPH蛋白进行体外验证。
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2. **《Structural characterization of MYBPH and its interaction with myosin heavy chain》**
Authors: Smith JL, et al.
**摘要**: 通过X射线晶体学解析重组人MYBPH蛋白结构，发现其羧基端含有α-螺旋结构域可直接结合肌球蛋白重链，提示该蛋白在调节肌纤维组装中的作用，为肌肉疾病机制提供结构生物学依据。
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3. **《Proteomic identification of MYBPH as a biomarker in non-small cell lung cancer》**
Authors: Chen H, et al.
**摘要**: 基于重组MYBPH蛋白的ELISA检测发现，其在非小细胞肺癌患者血清中显著高表达，且表达水平与淋巴结转移正相关，提示MYBPH可能作为肿瘤转移的潜在生物标志物。
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注：上述文献信息为示例性质，实际文献需通过PubMed/Web of Science查询确认。若需具体文献DOI或实验细节补充，请进一步说明。
**Recombinant Human MYBPH Protein: Background**
Myosin-binding protein H (MYBPH) is a component of the sarcomeric thick filaments in striated muscles, primarily known for its role in muscle structure and function. It belongs to a family of myosin-binding proteins that regulate myosin filament assembly, stability, and contractile activity. MYBPH interacts with myosin heavy chains and titin, contributing to sarcomere organization and modulating muscle mechanics. While its function is well-characterized in cardiac and skeletal muscles, recent studies suggest broader implications, including potential roles in cell motility and cancer progression. For instance, MYBPH has been linked to tumor invasiveness in head and neck squamous cell carcinoma, possibly through cytoskeletal remodeling pathways.
The recombinant form of MYBPH is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Its recombinant version retains key domains, such as immunoglobulin-like folds, critical for binding partners. Researchers utilize recombinant MYBPH to investigate its structural interactions, post-translational modifications, and disease-related mechanisms. Additionally, it serves as a tool for screening therapeutic agents targeting muscle disorders or cancers associated with MYBPH dysregulation. Despite progress, the full spectrum of its physiological and pathological roles remains an active area of exploration, emphasizing its dual significance in basic research and biomedical applications.
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