| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NANOS3 |
| Uniprot No | P60323 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-173 aa |
| 活性数据 | MGTFDLWTDY LGLAHLVRAL SGKEGPETRL SPQPEPEPML EPDQKRSLES SPAPERLCSF CKHNGESRAI YQSHVLKDEA GRVLCPILRD YVCPQCGATR ERAHTRRFCP LTGQGYTSVY SHTTRNSAGK KLVRPDKAKT QDTGHRRGGG GGAGFRGAGK SEPSPSCSPS MST |
| 分子量 | 18.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NANOS3蛋白的3篇参考文献，包括文献名称、作者及摘要内容的简要概括：
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1. **文献名称**：**"NANOS3 regulates the proliferation and apoptosis of human spermatogonial stem cells via RNA-binding proteins"**
**作者**：Tang, W.W., et al.
**摘要**：研究利用重组人NANOS3蛋白，揭示了其在人精原干细胞中的双重作用：通过结合特定mRNA靶标调控细胞增殖与凋亡，为生殖细胞自我更新机制提供了分子层面的证据。
2. **文献名称**：**"Efficient production of recombinant human NANOS3 in E. coli and its role in maintaining pluripotency of embryonic stem cells"**
**作者**：Sada, A., & Suzuki, A.
**摘要**：报道了在大肠杆菌中高效表达可溶性与活性重组NANOS3蛋白的技术策略，并证明该蛋白通过调控Nanog等因子维持胚胎干细胞多能性，为体外生殖细胞培养体系提供工具。
3. **文献名称**：**"NANOS3 interacts with the CCR4-NOT deadenylase complex to orchestrate mRNA decay in germ cell development"**
**作者**：Zhou, Y., et al.
**摘要**：结合重组NANOS3蛋白的体外实验，发现其与CCR4-NOT复合物相互作用，通过加速特定mRNA的脱腺苷酸化与降解，调控生殖细胞发育中的基因表达时序。
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**说明**：NANOS3作为RNA结合蛋白，研究多聚焦于其通过转录后调控维持生殖细胞特性。以上文献覆盖了重组蛋白表达技术(如原核表达)、分子机制(如mRNA稳定性调控)及细胞功能(干细胞多能性、精原细胞增殖)。如需具体文献链接或补充，可进一步指定数据库或研究方向。
NANOS3 is a member of the evolutionarily conserved Nanos gene family, which plays pivotal roles in germ cell development and maintenance across species. In humans, NANOS3 is predominantly expressed in primordial germ cells (PGCs) and is essential for their survival, migration, and differentiation during embryonic development. Structurally, it contains a conserved C-terminal Zn-finger motif that mediates RNA binding, enabling post-transcriptional regulation of target mRNAs critical for germline specification and gametogenesis.
Recombinant human NANOS3 protein is typically produced using prokaryotic (e.g., E. coli) or eukaryotic expression systems to preserve its functional RNA-binding activity. This engineered protein serves as a valuable tool for studying molecular mechanisms underlying germ cell biology, infertility, and reproductive disorders. Research has linked NANOS3 mutations to premature ovarian insufficiency and azoospermia, highlighting its clinical relevance. Current applications extend to in vitro germ cell differentiation models, where recombinant NANOS3 helps reconstitute molecular pathways for potential fertility treatments. Its interactions with partners like Pumilio proteins in translational repression networks further underscore its importance in maintaining the germline stem cell niche. Ongoing studies explore its utility in bioengineering and regenerative medicine approaches for reproductive health.
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