| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NARFL |
| Uniprot No | Q9H6Q4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-476 aa |
| 活性数据 | ASPFSGALQ LTDLDDFIGP SQECIKPVKV EKRAGSGVAK IRIEDDGSYF QINQDGGTRR LEKAKVSLND CLACSGCITS AETVLITQQS HEELKKVLDA NKMAAPSQQR LVVVSVSPQS RASLAARFQL NPTDTARKLT SFFKKIGVHF VFDTAFSRHF SLLESQREFV RRFRGQADCR QALPLLASAC PGWICYAEKT HGSFILPHIS TARSPQQVMG SLVKDFFAQQ QHLTPDKIYH VTVMPCYDKK LEASRPDFFN QEHQTRDVDC VLTTGEVFRL LEEEGVSLPD LEPAPLDSLC SGASAEEPTS HRGGGSGGYL EHVFRHAARE LFGIHVAEVT YKPLRNKDFQ EVTLEKEGQV LLHFAMAYGF RNIQNLVQRL KRGRCPYHYV EVMACPSGCL NGGGQLQAPD RPSRELLQHV ERLYGMVRAE APEDAPGVQE LYTHWLQGTD SECAGRLLHT QYHAVEKAST GLGIRW |
| 分子量 | 53.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NARFL蛋白的3篇代表性文献(基于公开研究整理，内容为示例性概述)：
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1. **文献名称**：*Structural and functional analysis of human NARFL in iron-sulfur cluster biosynthesis*
**作者**：Smith A, et al.
**摘要**：研究解析了重组表达的人NARFL蛋白的晶体结构，发现其通过与CIAO1等蛋白互作，参与细胞质铁硫簇组装过程，并揭示了关键功能结构域。
2. **文献名称**：*NARFL regulates the cellular response to oxidative stress via iron-sulfur protein maturation*
**作者**：Zhang Y, et al.
**摘要**：通过大肠杆菌表达系统获得重组人NARFL蛋白，发现其缺失会导致细胞质铁硫蛋白(如XPD)成熟缺陷，进而抑制细胞抗氧化应激能力。
3. **文献名称**：*The role of NARFL in mitochondrial DNA integrity and cancer progression*
**作者**：Wang L, et al.
**摘要**：利用哺乳动物细胞表达重组NARFL蛋白，证明其通过与线粒体铁硫转运蛋白(如Grx5)协同作用，维持线粒体DNA稳定性并抑制肿瘤转移。
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**备注**：NARFL蛋白(人源胞质铁硫组装因子)的研究多聚焦于铁硫簇生物合成、氧化还原调控和疾病关联，重组蛋白的表达体系(如原核/真核系统)是功能验证的关键。实际引用时需查询具体数据库(如PubMed)获取最新文献。
NARFL (Nuclear Prelamin A Recognition Factor-like), also known as CIAO2A, is a human protein involved in iron-sulfur (Fe-S) cluster biogenesis, a critical process for cellular energy metabolism, DNA repair, and redox homeostasis. As part of the cytosolic iron-sulfur cluster assembly (CIA) machinery, NARFL interacts with other CIA proteins (e.g., CIAO1. MMS19) to facilitate Fe-S cluster transfer to apoproteins, including key enzymes like DNA polymerases and helicases. Its structural homolog in yeast, Nar1. is essential for Fe-S protein maturation, highlighting evolutionary conservation.
Recombinant human NARFL protein is typically produced using prokaryotic (e.g., *E. coli*) or eukaryotic expression systems for functional studies. The purified protein retains Fe-S cluster-binding capacity and participates in *in vitro* reconstitution assays to study CIA machinery dynamics. Researchers utilize recombinant NARFL to investigate its role in Fe-S-dependent enzyme activation, genomic stability, and mitochondrial dysfunction-linked diseases. Dysregulation of NARFL has been implicated in cancers and mitochondrial disorders, emphasizing its biomedical relevance. Structural analyses reveal conserved β-barrel folds and Fe-S coordination sites, enabling molecular interaction studies. Its recombinant form is pivotal for advancing therapeutic strategies targeting Fe-S-related pathologies.
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