| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLF2 |
| Uniprot No | Q9Y5W3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-355aa |
| 氨基酸序列 | MALSEPILPS FSTFASPCRE RGLQERWPRA EPESGGTDDD LNSVLDFILS MGLDGLGAEA APEPPPPPPP PAFYYPEPGA PPPYSAPAGG LVSELLRPEL DAPLGPALHG RFLLAPPGRL VKAEPPEADG GGGYGCAPGL TRGPRGLKRE GAPGPAASCM RGPGGRPPPP PDTPPLSPDG PARLPAPGPR ASFPPPFGGP GFGAPGPGLH YAPPAPPAFG LFDDAAAAAA ALGLAPPAAR GLLTPPASPL ELLEAKPKRG RRSWPRKRTA THTCSYAGCG KTYTKSSHLK AHLRTHTGEK PYHCNWDGCG WKFARSDELT RHYRKHTGHR PFQCHLCDRA FSRSDHLALH MKRHM |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KLF2重组蛋白的3篇参考文献及其简要概括：
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1. **文献名称**：*"Krüppel-like Factor 2 (KLF2) Regulates T Cell Activation and Survival"*
**作者**：Kuo, C.T., et al.
**摘要**：该研究通过重组KLF2蛋白及过表达实验，揭示了KLF2在调节T细胞存活和归巢受体表达中的关键作用，表明其通过抑制促炎信号通路维持T细胞稳态。
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2. **文献名称**：*"KLF2 Is a Novel Transcriptional Regulator of Endothelial Proinflammatory Activation"*
**作者**：SenBanerjee, S., et al.
**摘要**：研究利用重组KLF2蛋白和基因沉默技术，证明KLF2通过抑制NF-κB通路显著降低内皮细胞炎症因子表达，提示其在抗动脉粥样硬化中的治疗潜力。
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3. **文献名称**：*"Regulation of Endothelial Cell Differentiation and Specification by KLF2"*
**作者**：Parmar, K.M., et al.
**摘要**：通过重组KLF2蛋白的功能实验，发现其通过调控Notch信号通路影响内皮细胞分化和血管生成，为血管发育异常相关疾病提供了分子机制解释。
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这些文献均涉及KLF2重组蛋白在免疫调节、炎症反应及血管生物学中的功能研究，涵盖实验模型中的表达、功能验证及潜在治疗应用。
KLF2 (Krüppel-like factor 2) recombinant protein is a lab-engineered version of the human transcription factor that plays critical roles in vascular homeostasis, immune regulation, and cellular differentiation. As a member of the Krüppel-like factor family, KLF2 contains three C-terminal zinc finger motifs enabling DNA binding and an N-terminal domain for transcriptional regulation. It is broadly expressed in endothelial cells, lymphocytes, and adipocytes, where it modulates genes involved in inflammation (e.g., NF-κB suppression), oxidative stress response, and vascular tone (e.g., NOS3 activation).
The recombinant protein is typically produced using bacterial (E. coli) or mammalian expression systems, followed by purification via affinity tags like His-tag. Its production enables mechanistic studies of KLF2's biological functions without interference from endogenous cellular pathways. Researchers frequently employ it to investigate KLF2-mediated protection against atherosclerosis, as it enhances endothelial barrier integrity and reduces vascular adhesion molecule expression. In immunology, KLF2 recombinant protein helps elucidate its role in T-cell quiescence and thymocyte survival through interactions with transcription factors like Foxp3.
Pharmaceutically, KLF2 is explored as a therapeutic target for cardiovascular diseases and autoimmune disorders. Its recombinant form serves as a tool for drug screening, particularly for compounds mimicking its anti-inflammatory effects under shear stress conditions. Paradoxically, KLF2 also exhibits context-dependent oncogenic or tumor-suppressive activities in cancers, necessitating further research using purified protein for pathway validation. Current challenges include optimizing its stability for in vivo applications and understanding post-translational modifications affecting its activity.
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