| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NCAPH |
| Uniprot No | Q15003 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-741 aa |
| 活性数据 | MGPPGPALPA TMNNSSSETR GHPHSASSPS ERVFPMPLPR KAPLNIPGTP VLEDFPQNDD EKERLQRRRS RVFDLQFSTD SPRLLASPSS RSIDISATIP KFTNTQITEH YSTCIKLSTE NKITTKNAFG LHLIDFMSEI LKQKDTEPTN FKVAAGTLDA STKIYAVRVD AVHADVYRVL GGLGKDAPSL EEVEGHVADG SATEMGTTKK AVKPKKKHLH RTIEQNINNL NVSEADRKCE IDPMFQKTAA SFDECSTAGV FLSTLHCQDY RSELLFPSDV QTLSTGEPLE LPELGCVEMT DLKAPLQQCA EDRQICPSLA GFQFTQWDSE THNESVSALV DKFKKNDQVF DINAEVDESD CGDFPDGSLG DDFDANDEPD HTAVGDHEEF RSWKEPCQVQ SCQEEMISLG DGDIRTMCPL LSMKPGEYSY FSPRTMSMWA GPDHWRFRPR RKQDAPSQSE NKKKSTKKDF EIDFEDDIDF DVYFRKTKAA TILTKSTLEN QNWRATTLPT DFNYNVDTLV QLHLKPGTRL LKMAQGHRVE TEHYEEIEDY DYNNPNDTSN FCPGLQAADS DDEDLDDLFV GPVGNSDLSP YPCHPPKTAQ QNGDTPEAQG LDITTYGESN LVAEPQKVNK IEIHYAKTAK KMDMKKLKQS MWSLLTALSG KEADAEANHR EAGKEAALAE VADEKMLSGL TKDLQRSLPP VMAQNLSIPL AFACLLHLAN EKNLKLEGTE DLSDVLVRQG D |
| 分子量 | 82.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NCAPH蛋白的3篇参考文献概览：
1. **文献名称**：NCAPH plays important roles in lung cancer
**作者**：Zhu et al. (2020)
**摘要**：该研究揭示NCAPH在非小细胞肺癌中高表达，通过调控细胞周期蛋白(如Cyclin B1)促进癌细胞增殖和转移，提示其作为潜在治疗靶点。
2. **文献名称**：Condensin subunit NCAPH2 regulates mitotic chromosome architecture
**作者**：Hirota et al. (2016)
**摘要**：研究利用CRISPR-Cas9筛选和活细胞成像，发现NCAPH是凝缩蛋白复合体的关键组分，在维持有丝分裂染色体正确结构和基因组稳定性中不可或缺。
3. **文献名称**：NCAPH as a prognostic biomarker in breast cancer
**作者**：Wang et al. (2021)
**摘要**：分析乳腺癌患者数据发现，NCAPH高表达与不良预后相关，可能通过促进上皮-间质转化(EMT)驱动肿瘤侵袭，提出其作为预后标志物的潜力。
这些研究覆盖了NCAPH在染色体结构维持、癌症发展及临床预后中的多重作用。如需具体文献标题或补充更多研究，可进一步访问PubMed等数据库检索。
NCAPH (Non-SMC Condensin I Complex Subunit H) is a key component of the condensin I complex, essential for chromosome condensation and genomic stability during mitosis and meiosis. As part of the conserved SMC (Structural Maintenance of Chromosomes) protein family, condensin I ensures proper chromatin organization by compressing chromosomal DNA into distinct, manageable structures. NCAPH binds directly to the SMC2/SMC4 heterodimer, stabilizing the complex and enabling its ATP-dependent activity in loop formation and chromatin compaction. Its function is critical during cell division, particularly in prophase, to prevent chromosomal entanglements and ensure faithful sister chromatid segregation.
Dysregulation of NCAPH is linked to genomic instability and aneuploidy, hallmarks of cancer. Overexpression of NCAPH has been observed in multiple malignancies, including colorectal, breast, and liver cancers, where it correlates with poor prognosis, tumor progression, and chemoresistance. Studies suggest NCAPH promotes oncogenesis by enhancing cell proliferation, inhibiting apoptosis, and facilitating epithelial-mesenchymal transition.
Recombinant human NCAPH protein, typically produced in *E. coli* or mammalian expression systems, enables *in vitro* studies of condensin I’s biochemical properties, chromatin remodeling mechanisms, and interactions with DNA or other regulatory factors. It is widely used in structural biology, enzymatic assays, and drug discovery targeting mitotic errors or cancer therapeutics. Research on recombinant NCAPH also aids in elucidating its role in developmental disorders linked to chromosomal missegregation.
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