| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NCOA4 |
| Uniprot No | Q13772 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-614 aa |
| 活性数据 | MNTFQDQSGS SSNREPLLRC SDARRDLELA IGGVLRAEQQ IKDNLREVKA QIHSCISRHL ECLRSREVWL YEQVDLIYQL KEETLQQQAQ QLYSLLGQFN CLTHQLECTQ NKDLANQVSV CLERLGSLTL KPEDSTVLLF EADTITLRQT ITTFGSLKTI QIPEHLMAHA SSANIGPFLE KRGCISMPEQ KSASGIVAVP FSEWLLGSKP ASGYQAPYIP STDPQDWLTQ KQTLENSQTS SRACNFFNNV GGNLKGLENW LLKSEKSSYQ KCNSHSTTSS FSIEMEKVGD QELPDQDEMD LSDWLVTPQE SHKLRKPENG SRETSEKFKL LFQSYNVNDW LVKTDSCTNC QGNQPKGVEI ENLGNLKCLN DHLEAKKPLS TPSMVTEDWL VQNHQDPCKV EEVCRANEPC TSFAECVCDE NCEKEALYKW LLKKEGKDKN GMPVEPKPEP EKHKDSLNMW LCPRKEVIEQ TKAPKAMTPS RIADSFQVIK NSPLSEWLIR PPYKEGSPKE VPGTEDRAGK QKFKSPMNTS WCSFNTADWV LPGKKMGNLS QLSSGEDKWL LRKKAQEVLL NSPLQEEHNF PPDHYGLPAV CDLFACMQLK VDKEKWLYRT PLQM |
| 分子量 | 69.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NCOA4蛋白的3篇关键文献概述，涵盖其功能机制与疾病关联：
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1. **文献名称**：*Ferritinophagy via NCOA4 is required for cellular iron homeostasis*
**作者**：Mancias, J.D., Wang, X., Gygi, S.P., et al.
**期刊/年份**：*Nature* (2014)
**摘要**：首次阐明NCOA4作为选择性自噬受体，通过介导铁蛋白(ferritin)的自噬降解(铁自噬)调节细胞内铁离子稳态，揭示其在铁代谢中的核心作用。
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2. **文献名称**：*NCOA4 links androgen receptor activation and castration-resistant prostate cancer*
**作者**：Gannon, P.O., Medelci, S., Le Page, C., et al.
**期刊/年份**：*Cancer Research* (2013)
**摘要**：研究揭示NCOA4通过增强雄激素受体(AR)的转录活性促进去势抵抗性前列腺癌进展，提示其作为癌症治疗潜在靶点。
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3. **文献名称**：*NCOA4-dependent ferritinophagy is a key regulator of mitochondrial reactive oxygen species*
**作者**：Santana-Codina, N., Gikandi, A., Mancias, J.D.
**期刊/年份**：*Molecular Cell* (2021)
**摘要**：提出NCOA4介导的铁自噬通过调控线粒体铁水平影响活性氧(ROS)生成，连接铁代谢与氧化应激机制，为相关疾病提供新视角。
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**说明**：以上文献围绕NCOA4在铁代谢、癌症及氧化应激中的功能，建议通过PubMed或期刊官网获取全文验证细节。
**Background of Recombinant Human NCOA4 Protein**
NCOA4 (Nuclear Receptor Coactivator 4) is a multifunctional protein implicated in critical cellular processes, including selective autophagy and iron metabolism. Initially identified as a coactivator for nuclear receptors, it is now recognized as a key autophagy receptor mediating the degradation of specific cargo, most notably ferritin—a major iron-storage complex. This process, termed ferritinophagy, involves NCOA4 binding to ferritin and targeting it to autophagosomes for lysosomal degradation, thereby releasing iron to maintain cellular iron homeostasis.
Structurally, NCOA4 contains coiled-coil domains and a C-terminal region critical for ferritin interaction. It exists in multiple isoforms, with tissue-specific expression patterns, particularly abundant in tissues with high iron demand like the liver and spleen. Dysregulation of NCOA4 is linked to pathologies, including iron overload disorders, neurodegenerative diseases (e.g., Alzheimer’s), and cancers (e.g., prostate and hepatocellular carcinoma), where its aberrant expression affects iron-dependent processes like proliferation and oxidative stress.
Recombinant NCOA4 protein, generated via bacterial or mammalian expression systems, serves as a vital tool for studying ferritinophagy mechanisms, protein-protein interactions, and therapeutic targeting. Its role in iron metabolism and connections to ferroptosis—an iron-dependent cell death pathway—further underscore its biomedical relevance, driving interest in modulating NCOA4 activity for disease intervention.
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