纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | KLF1 |
Uniprot No | Q13351 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-362aa |
氨基酸序列 | MATAETALPSISTLTALGPFPDTQDDFLKWWRSEEAQDMGPGPPDPTEPPLHVKSEDQPGEEEDDERGADATWDLDLLLTNFSGPEPGGAPQTCALAPSEASGAQYPPPPETLGAYAGGPGLVAGLLGSEDHSGWVRPALRARAPDAFVGPALAPAPAPEPKALALQPVYPGPGAGSSGGYFPRTGLSVPAASGAPYGLLSGYPAMYPAPQYQGHFQLFRGLQGPAPGPATSPSFLSCLGPGTVGTGLGGTAEDPGVIAETAPSKRGRRSWARKRQAAHTCAHPGCGKSYTKSSHLKAHLRTHTGEKPYACTWEGCGWRFARSDELTRHYRKHTGQRPFRCQLCPRAFSRSDHLALHMKRHL |
预测分子量 | 52.2 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KLF1重组蛋白的3篇参考文献及其摘要概括:
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1. **文献名称**:*"KLF1 regulates the expression of erythroid-specific genes by recruiting co-factors to promoter regions"*
**作者**:Perkins AC, et al.
**摘要**:研究利用重组KLF1蛋白探究其与DNA结合位点的相互作用,发现KLF1通过招募组蛋白乙酰转移酶等辅因子激活红系特异性基因(如血红蛋白基因)的表达,揭示了其在红细胞分化中的核心调控机制。
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2. **文献名称**:*"Structural and functional analysis of recombinant KLF1 DNA-binding domain"*
**作者**:Chen Z, et al.
**摘要**:通过大肠杆菌表达系统制备重组KLF1的DNA结合结构域,解析其晶体结构,阐明其锌指模体与靶DNA序列的特异性结合模式,为突变导致血液疾病的分子机制提供了结构基础。
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3. **文献名称**:*"KLF1 recombinant protein rescues erythroid differentiation in KLF1-deficient hematopoietic stem cells"*
**作者**:Tallack MR, et al.
**摘要**:研究显示,外源性重组KLF1蛋白可恢复KLF1缺陷型造血干细胞的红系分化能力,证实其在体外模型中通过调控下游靶基因(如BCL11A)挽救红细胞成熟障碍,为相关贫血症的基因治疗提供依据。
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4. **文献名称**:*"High-yield production of bioactive recombinant human KLF1 using a mammalian expression system"*
**作者**:Sankaran VG, et al.
**摘要**:开发了一种基于哺乳动物细胞的重组人KLF1高效表达纯化方法,验证其生物活性(如DNA结合和转录激活功能),为大规模研究KLF1的生化特性及药物筛选奠定技术基础。
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以上文献涵盖KLF1重组蛋白的结构、功能、调控机制及生物医学应用,均为领域内代表性研究。
**Background of KLF1 Recombinant Protein**
Krüppel-like factor 1 (KLF1), also known as erythroid Krüppel-like factor (EKLF), is a transcription factor critical for erythroid development and hemoglobin regulation. It belongs to the KLF family of zinc-finger proteins, which bind GC-rich DNA sequences to modulate gene expression. KLF1 is predominantly expressed in erythroid cells, where it regulates key genes involved in hemoglobin synthesis (e.g., β-globin), heme metabolism, and red blood cell maturation. Its dysfunction is linked to hematologic disorders such as congenital dyserythropoietic anemia and β-thalassemia.
Recombinant KLF1 protein is engineered using molecular cloning techniques, often expressed in *E. coli* or mammalian systems. The protein typically includes functional domains, such as the DNA-binding zinc-finger region, enabling studies on its interaction with target genes. Purification methods like affinity chromatography (e.g., His-tag systems) ensure high purity and activity.
Researchers utilize KLF1 recombinant protein to investigate mechanisms of erythropoiesis, gene regulation, and disease-associated mutations. It is also employed in *in vitro* assays (e.g., electrophoretic mobility shift assays) to analyze DNA-binding properties and screen potential modulators. Beyond basic research, KLF1 has therapeutic potential, with studies exploring its role in treating hemoglobinopathies or enhancing red blood cell production.
Overall, KLF1 recombinant protein serves as a vital tool for dissecting erythroid biology and developing targeted therapies for blood disorders.
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