Recombinant Human KLF1 protein

**Background of KLF1 Recombinant Protein**

Krüppel-like factor 1 (KLF1), also known as erythroid Krüppel-like factor (EKLF), is a transcription factor critical for erythroid development and hemoglobin regulation. It belongs to the KLF family of zinc-finger proteins, which bind GC-rich DNA sequences to modulate gene expression. KLF1 is predominantly expressed in erythroid cells, where it regulates key genes involved in hemoglobin synthesis (e.g., β-globin), heme metabolism, and red blood cell maturation. Its dysfunction is linked to hematologic disorders such as congenital dyserythropoietic anemia and β-thalassemia.

Recombinant KLF1 protein is engineered using molecular cloning techniques, often expressed in *E. coli* or mammalian systems. The protein typically includes functional domains, such as the DNA-binding zinc-finger region, enabling studies on its interaction with target genes. Purification methods like affinity chromatography (e.g., His-tag systems) ensure high purity and activity.

Researchers utilize KLF1 recombinant protein to investigate mechanisms of erythropoiesis, gene regulation, and disease-associated mutations. It is also employed in *in vitro* assays (e.g., electrophoretic mobility shift assays) to analyze DNA-binding properties and screen potential modulators. Beyond basic research, KLF1 has therapeutic potential, with studies exploring its role in treating hemoglobinopathies or enhancing red blood cell production.

Overall, KLF1 recombinant protein serves as a vital tool for dissecting erythroid biology and developing targeted therapies for blood disorders.