| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NCOA6IP |
| Uniprot No | Q96RS0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 713-853 aa |
| 活性数据 | MRVIAIDIDPVKIALARNNAEVYGIADKIEFICGDFLLLASFLKADVVFLSPPWGGPDYATAETFDIRTMMSPDGFEIFRLSKKITNNIVYFLPRNADIDQVASLAGPGGQVEIEQNFLNNKLKTITAYFGDLIRRPASET |
| 分子量 | 31.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Cloning and characterization of NCOA6IP, a novel nuclear receptor coactivator"**
*Authors: Qin C, et al.*
摘要：该研究克隆并鉴定了人NCOA6IP蛋白，发现其作为核受体共激活因子，与NCOA6相互作用，参与配体依赖性转录调控，并解析了其重组蛋白的基本结构与功能。
2. **"Functional analysis of PRO2000/UKp68 as a mediator of nuclear receptor signaling"**
*Authors: Lee YH, et al.*
摘要：研究重组表达NCOA6IP(PRO2000)蛋白，证实其通过桥接核受体与染色质重塑复合物，增强甲状腺激素受体(TR)等核受体的转录活性。
3. **"NCOA6IP links mitochondrial dysfunction to human diseases"**
*Authors: Smith J, et al.*
摘要：通过重组蛋白实验发现NCOA6IP在线粒体RNA加工中的潜在作用，异常表达可能与神经退行性疾病相关，为疾病机制研究提供新方向。
4. **"Structural insights into NCOA6IP-mediated transcriptional regulation"**
*Authors: Zhang L, et al.*
摘要：利用重组人NCOA6IP蛋白进行结构生物学分析，揭示其通过C端结构域与核受体结合，并参与形成多蛋白复合物以调控靶基因表达。
(注：以上文献标题及作者为示例性虚拟内容，实际需通过PubMed/Google Scholar检索具体文献。)
Nuclear receptor coactivator 6-interacting protein (NCOA6IP), also known as ERAP1 or PSA, is a multifunctional protein implicated in transcriptional regulation and mitochondrial metabolism. It was initially identified as a binding partner of nuclear receptor coactivator 6 (NCOA6), facilitating transcriptional activation of hormone receptors and other transcription factors. Structurally, it contains conserved domains, including an RNA-recognition motif (RRM) and zinc finger motifs, which enable RNA binding and protein interactions.
NCOA6IP exhibits dual subcellular localization: in the nucleus, it modulates gene expression by bridging transcription factors with chromatin-modifying complexes; in mitochondria, it interacts with mitochondrial tRNA-processing enzymes and respiratory chain components, suggesting roles in mitochondrial RNA homeostasis and energy metabolism. Its mitochondrial involvement links it to cellular stress responses and metabolic regulation.
Dysregulation of NCOA6IP is associated with pathologies such as cancer, metabolic disorders, and neurodegenerative diseases. For instance, somatic mutations in NCOA6IP correlate with altered mitochondrial function in tumors, while its reduced expression is observed in type 2 diabetes models. Recent studies also highlight its interaction with viral proteins, indicating potential roles in host-pathogen interactions.
Though its precise molecular mechanisms remain under investigation, NCOA6IP’s dual localization and multifunctional nature position it as a critical node in integrating nuclear transcriptional programs with mitochondrial energetics, offering therapeutic targets for metabolic and degenerative diseases.
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