| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NDUFA9 |
| Uniprot No | Q16795 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 36-377 aa |
| 活性数据 | LHHAL MPHGKGGRSS VSGIVATVFG ATGFLGRYVV NHLGRMGSQV IIPYRCDKYD IMHLRPMGDL GQLLFLEWDA RDKDSIRRVV QHSNVVINLI GRDWETKNFD FEDVFVKIPQ AIAQLSKEAG VEKFIHVSHL NANIKSSSRY LRNKAVGEKV VRDAFPEAII VKPSDIFGRE DRFLNSFASM HRFGPIPLGS LGWKTVKQPV YVVDVSKGIV NAVKDPDANG KSFAFVGPSR YLLFHLVKYI FAVAHRLFLP FPLPLFAYRW VARVFEISPF EPWITRDKVE RMHITDMKLP HLPGLEDLGI QATPLELKAI EVLRRHRTYR WLSAEIEDVK PAKTVNI |
| 分子量 | 42.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条假设的关于“重组人NDUFA9蛋白”的参考文献示例(仅供格式参考，非真实文献)：
1. **《重组人NDUFA9蛋白的克隆与原核表达研究》**
- **作者**：Smith J, Brown L.
- **摘要**：研究报道了通过PCR扩增人源NDUFA9基因，成功构建其原核表达载体，并利用大肠杆菌系统实现了重组蛋白的高效表达。通过亲和层析纯化获得了高纯度NDUFA9蛋白，为后续功能研究提供了基础。
2. **《NDUFA9在线粒体复合体I中的功能及其结构解析》**
- **作者**：Zhang Y, et al.
- **摘要**：通过重组人NDUFA9蛋白与复合体I其他亚基的共表达实验，揭示了其在电子传递链中稳定复合体结构的关键作用。结合突变分析，证明NDUFA9缺失会导致复合体I酶活性显著下降。
3. **《NDUFA9基因突变与线粒体脑肌病的关联研究》**
- **作者**：Wang R, et al.
- **摘要**：利用重组NDUFA9蛋白模拟患者突变体，发现其与复合体I装配异常直接相关。细胞实验表明，突变蛋白导致线粒体膜电位降低，为疾病机制提供了分子依据。
4. **《重组NDUFA9蛋白的晶体结构及辅酶结合位点分析》**
- **作者**：Johnson M, et al.
- **摘要**：通过X射线晶体学解析了重组NDUFA9蛋白的三维结构，揭示了其与辅酶Q结合的活性区域。该研究为开发靶向复合体I的药物提供了结构基础。
(注：以上为模拟文献，实际研究中请通过PubMed或Google Scholar等平台检索真实发表的论文。)
The recombinant human NDUFA9 protein is a key subunit of mitochondrial Complex I (NADH:ubiquinone oxidoreductase), the largest enzyme in the electron transport chain responsible for oxidative phosphorylation. NDUFA9. encoded by the nuclear gene NDUFA9. is a 39 kDa iron-sulfur (Fe-S) cluster-binding protein that contributes to Complex I assembly, stability, and electron transfer activity. It contains a conserved NADH-binding domain and interacts with other core subunits to mediate the transfer of electrons from NADH to ubiquinone, driving proton translocation across the mitochondrial inner membrane.
Recombinant NDUFA9 is typically produced using bacterial or mammalian expression systems, enabling studies on its structural and functional roles in health and disease. Mutations in NDUFA9 are linked to mitochondrial disorders, such as Leigh syndrome and other Complex I deficiencies, characterized by impaired ATP production and neurodegenerative symptoms. Research using recombinant NDUFA9 aids in elucidating pathogenic mechanisms, screening therapeutic compounds, and developing gene therapy strategies. Additionally, it serves as a critical tool for structural studies (e.g., cryo-EM) to map Complex I architecture and investigate redox-driven conformational changes. Its application extends to exploring metabolic reprogramming in cancer and age-related mitochondrial dysfunction.
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