| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NDUFB1 |
| Uniprot No | O75438 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-58 aa |
| 活性数据 | VNLLQIVRDHWVHVLVPMGFVIGCYLDRKSDERLTAFRNKSMLFKRELQPSEEVTWK |
| 分子量 | 33.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NDUFB1蛋白的3篇参考文献示例(内容为虚构，仅供格式参考)：
1. **标题**: "Recombinant human NDUFB1 expression and its role in mitochondrial complex I assembly"
**作者**: Smith J, et al.
**摘要**: 研究在大肠杆菌中成功表达重组人NDUFB1蛋白，验证其参与线粒体复合物I的组装过程，并通过敲低实验证明其缺失导致细胞能量代谢缺陷。
2. **标题**: "Structural characterization of NDUFB1 in the context of respiratory chain supercomplexes"
**作者**: Chen L, et al.
**摘要**: 利用冷冻电镜解析含重组NDUFB1的复合物I结构，揭示其跨膜结构域在电子传递中的关键作用，并探讨突变位点对神经退行性疾病的影响。
3. **标题**: "NDUFB1 mutations linked to Leigh syndrome impair recombinant protein stability"
**作者**: Gupta R, et al.
**摘要**: 通过构建NDUFB1突变体重组蛋白，发现特定致病突变导致其无法整合到复合物I中，为Leigh综合征的分子机制提供实验依据。
**提示**：以上为模拟文献，实际研究请通过PubMed/SciHub等平台以"NDUFB1"、"recombinant protein"、"complex I"为关键词检索。
**Background of Recombinant Human (NDUFB1) Protein**
NDUFB1 (NADH:ubiquinone oxidoreductase subunit B1) is a nuclear-encoded protein component of mitochondrial respiratory chain Complex I (NADH dehydrogenase), which catalyzes the first step in the electron transport chain, transferring electrons from NADH to ubiquinone. This process drives proton translocation across the mitochondrial inner membrane, contributing to ATP synthesis. As a subunit of the hydrophobic "membrane arm" of Complex I, NDUFB1 plays a structural and functional role in maintaining complex assembly, stability, and activity.
Recombinant human NDUFB1 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) for in vitro studies. It is often tagged (e.g., His-tag) to facilitate purification and detection. Dysregulation or mutations in NDUFB1 are linked to mitochondrial disorders, such as Leigh syndrome, and have been implicated in neurodegenerative diseases, cancer, and metabolic syndromes. Research on recombinant NDUFB1 aids in elucidating Complex I dysfunction mechanisms, screening disease-associated mutations, and exploring therapeutic targets. Its application extends to structural studies, antibody development, and biochemical assays to dissect mitochondrial energy metabolism pathways.
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