| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NECAB1 |
| Uniprot No | Q8N987 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-351 aa |
| 活性数据 | MEDSQETSPS SNNSSEELSS ALHLSKGMSI FLDILRRADK NDDGKLSFEE FKAYFADGVL SGEELHELFH TIDTHNTNNL DTEELCEYFS QHLGEYENVL AALEDLNLSI LKAMGKTKKD YQEASNLEQF VTRFLLKETL NQLQSLQNSL ECAMETTEEQ TRQERQGPAK PEVLSIQWPG KRSSRRVQRH NSFSPNSPQF NVSGPGLLEE DNQWMTQINR LQKLIDRLEK KDLKLEPPEE EIIEGNTKSH IMLVQRQMSV IEEDLEEFQL ALKHYVESAS SQSGCLRISI QKLSNESRYM IYEFWENSSV WNSHLQTNYS KTFQRSNVDF LETPELTSTM LVPASWWILN N |
| 分子量 | 40.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NECAB1蛋白的虚构参考文献示例，格式包含文献名称、作者及摘要内容概要：
---
1. **文献名称**: *"Expression and calcium-binding characterization of recombinant human NECAB1 in Escherichia coli"*
**作者**: Smith A.J., et al.
**摘要**: 本研究成功在大肠杆菌系统中表达了重组人NECAB1蛋白，并通过亲和层析纯化获得高纯度蛋白。体外实验证实其通过EF-hand结构域结合钙离子，为后续功能研究提供了基础工具。
2. **文献名称**: *"NECAB1 modulates synaptic plasticity via interaction with neuronal calcium sensor proteins"*
**作者**: Chen L., Wang H.
**摘要**: 发现NECAB1与突触钙传感器蛋白(如突触结合蛋白)相互作用，调节神经元内钙信号传导，影响突触可塑性和记忆相关通路，提示其在神经系统中的潜在功能。
3. **文献名称**: *"Structural insights into the EF-hand domain organization of NECAB1 by X-ray crystallography"*
**作者**: Tanaka K., et al.
**摘要**: 通过X射线晶体学解析了NECAB1蛋白的N端EF-hand结构域的三维结构，揭示了其钙离子结合位点的独特构象，为解释其与其他蛋白的相互作用机制提供了结构基础。
4. **文献名称**: *"Altered NECAB1 expression in Alzheimer's disease models: Implications for calcium dysregulation"*
**作者**: Müller R., et al.
**摘要**: 在阿尔茨海默病小鼠模型中观察到NECAB1的表达水平显著下降，可能导致神经元钙稳态失衡及突触功能障碍，提示其与神经退行性病变的潜在关联。
---
注：以上文献为模拟内容，实际研究中需通过学术数据库检索并验证。如需真实文献，建议使用关键词在PubMed或Web of Science中搜索。
NECAB1 (N-terminal EF-hand calcium-binding protein 1) is a calcium-binding protein belonging to the EF-hand superfamily, characterized by its conserved helix-loop-helix structural motifs that enable calcium ion coordination. Primarily expressed in neuronal tissues, NECAB1 is enriched in the brain, particularly in regions like the hippocampus, cortex, and striatum, suggesting roles in calcium-mediated signaling pathways critical for synaptic plasticity, neurotransmitter release, and neuronal development. It interacts with proteins involved in vesicle trafficking, such as synaptobrevin/VAMP2. and modulates presynaptic functions by regulating calcium dynamics. NECAB1 also associates with membrane receptors, including dopamine receptors, and influences downstream signaling cascades. Beyond neuroscience, it has been implicated in cancer, where dysregulated expression correlates with tumor progression and metastasis. Recombinant human NECAB1 protein is widely employed in biochemical studies to explore its calcium-dependent conformational changes, interaction networks, and potential as a therapeutic target. Its conserved structure across vertebrates underscores its functional importance, yet detailed mechanisms linking its calcium-sensing ability to cellular outcomes remain an active research area. This protein’s dual role in physiological and pathological contexts makes it a compelling subject for interdisciplinary studies spanning neurobiology, oncology, and molecular pharmacology.
×
