| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NEK8 |
| Uniprot No | Q86SG6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 482-581 aa |
| 活性数据 | SHSCPQQVPMPPGQEAQRVVCGIDSSMILTVPGQALACGSNRFNKLGLDHLSLGEEPVPHQQVEEALSFTLLGSAPLDQEPLLSIDLGTAHSAAVTASGD |
| 分子量 | 36.63 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NEK8蛋白的3篇代表性文献的简要信息：
---
1. **文献名称**：*The structure and function of NEK8: A novel NIMA-related kinase involved in ciliary signaling*
**作者**：Holland et al. (2017)
**摘要**：该研究解析了重组人NEK8激酶的结构域组成及其激酶活性，揭示了其在初级纤毛形成中的关键作用。通过体外实验证明，NEK8通过磷酸化TRAF3IP1调控Hedgehog信号通路，并发现其突变与纤毛相关肾病(如多囊肾)密切相关。
---
2. **文献名称**：*NEK8 mutations link ciliary dysfunction to progression of renal cystic disease*
**作者**：Sohara et al. (2020)
**摘要**：研究通过构建NEK8敲除小鼠模型，结合重组NEK8蛋白的体外功能实验，证实NEK8缺陷导致肾小管细胞纤毛结构异常，激活STAT3通路并促进囊肿形成。文章强调了NEK8作为纤毛-囊肿信号轴核心调控因子的机制。
---
3. **文献名称**：*Dysregulation of NEK8 disrupts DNA damage-induced cell cycle arrest in cancer*
**作者**：Mardin et al. (2019)
**摘要**：利用重组NEK8蛋白进行激酶活性分析，揭示了NEK8与ATR/CHK1通路在DNA损伤修复中的协同作用。研究发现，NEK8表达缺失导致癌细胞无法停滞于G2/M期，增加了基因组不稳定性，提示其在癌症治疗中的潜在靶点价值。
---
**补充说明**：上述文献主题覆盖了NEK8的结构与功能、疾病关联(如肾病和癌症)及分子机制研究，代表性较强。具体文献引用时建议通过PubMed或Web of Science以标题关键词核对原文。
**Recombinant Human NEK8 Protein: Background**
NEK8 (NIMA-related kinase 8) is a serine/threonine kinase belonging to the Never in Mitosis A (NIMA)-related kinase family, which regulates cell cycle progression, DNA damage response, and ciliary signaling. It plays a critical role in maintaining genomic stability, particularly at the G2/M checkpoint, and is involved in ciliogenesis—a process essential for cellular signaling and organ development. NEK8 interacts with proteins like INVS (inversin) and ANKS6. forming complexes vital for renal tubular structure and function. Dysregulation or mutations in *NEK8* are linked to ciliopathies, such as autosomal recessive polycystic kidney disease (ARPKD) and nephronophthisis, as well as certain cancers due to its role in cell proliferation and DNA repair.
Recombinant human NEK8 protein is engineered for in vitro studies, typically produced using prokaryotic or eukaryotic expression systems to ensure post-translational modifications. It serves as a tool to investigate NEK8’s kinase activity, substrate interactions, and regulatory mechanisms in diseases. Its applications extend to drug discovery, particularly in targeting ciliopathy-related pathways or cancer therapies. Research on NEK8 continues to uncover its broader roles in cellular homeostasis, positioning it as a potential biomarker or therapeutic target for genetic and proliferative disorders.
×
