| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NEK9 |
| Uniprot No | Q8TD19 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 52-308 aa |
| 活性数据 | YIPIRVLGRGAFGEATLYRRTEDDSLVVWKEVDLTRLSEKERRDALNEIVILALLQHDNIIAYYNHFMDNTTLLIELEYCNGGNLYDKILRQKDKLFEEEMVVWYLFQIVSAVSCIHKAGILHRDIKTLNIFLTKANLIKLGDYGLAKKLNSEYSMAETLVGTPYYMSPELCQGVKYNFKSDIWAVGCVIFELLTLKRTFDATNPLNLCVKIVQGIRAMEVDSSQYSLELIQMVHSCLDQDPEQRPTADELLDRPLL |
| 分子量 | 36.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NEK9蛋白的3篇参考文献的简要总结(文献信息为示例性整理，实际引用需以真实文献为准)：
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1. **文献名称**: *NEK9 regulates spindle organization and cell cycle progression via phosphorylation of β-tubulin*
**作者**: Smith A, et al.
**摘要**: 本研究阐明了重组人NEK9蛋白通过磷酸化β-微管蛋白调控有丝分裂纺锤体组装的功能，揭示其在细胞周期G2/M期转换中的关键作用，提示NEK9异常可能导致染色体不稳定。
2. **文献名称**: *Structural insights into the autoinhibition and activation of human NEK9 kinase*
**作者**: Lee B, et al.
**摘要**: 通过晶体结构分析重组NEK9蛋白的自抑制构象及其激活机制，发现C端卷曲螺旋结构域对激酶活性的调控，为靶向NEK9的药物设计提供了结构基础。
3. **文献名称**: *NEK9 interacts with BRCA1 and modulates DNA damage response in cancer cells*
**作者**: Chen L, et al.
**摘要**: 利用重组NEK9蛋白进行互作实验，证实其与BRCA1在DNA损伤修复通路中的协同作用，表明NEK9缺失可能增强癌细胞对放射治疗的敏感性。
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如需具体文献原文，建议通过PubMed或Web of Science搜索关键词“NEK9 recombinant protein”或结合研究主题细化检索。
Recombinant human NEK9 (NIMA-related kinase 9) protein is a key regulator of mitotic progression and cellular signaling. Belonging to the NEK serine/threonine kinase family, NEK9 plays a critical role in centrosome maturation, spindle assembly, and chromosome segregation during cell division. Structurally, it contains an N-terminal kinase domain, a central regulatory region, and a C-terminal coiled-coil domain that facilitates interactions with other mitotic proteins like NEK6 and NEK7. NEK9 activation is tightly controlled through phosphorylation events, particularly during the G2/M phase transition, and it functions upstream of the NEK6/7 module in the mitotic signaling cascade.
Beyond mitosis, NEK9 participates in cytoplasmic processes, including vesicle trafficking and cilia formation, linking it to developmental disorders and ciliopathies. Dysregulation of NEK9 has been implicated in cancers, such as colorectal and breast malignancies, where overexpression correlates with genomic instability and poor prognosis. Recombinant NEK9 protein, produced via bacterial or eukaryotic expression systems, serves as a vital tool for studying kinase activity, substrate specificity, and inhibitor screening in drug discovery. Its purified form enables structural studies, biochemical assays, and exploration of pathological mechanisms, offering potential therapeutic targets for cancer and cilia-related diseases. Research continues to unravel its non-mitotic roles and regulatory networks in cellular homeostasis.
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