| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NELL1 |
| Uniprot No | Q92832 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-810 aa |
| 活性数据 | FGMDPDLQM DIVTELDLVN TTLGVAQVSG MHNASKAFLF QDIEREIHAA PHVSEKLIQL FRNKSEFTIL ATVQQKPSTS GVILSIRELE HSYFELESSG LRDEIRYHYI HNGKPRTEAL PYRMADGQWH KVALSVSASH LLLHVDCNRI YERVIDPPDT NLPPGINLWL GQRNQKHGLF KGIIQDGKII FMPNGYITQC PNLNHTCPTC SDFLSLVQGI MDLQELLAKM TAKLNYAETR LSQLENCHCE KTCQVSGLLY RDQDSWVDGD HCRNCTCKSG AVECRRMSCP PLNCSPDSLP VHIAGQCCKV CRPKCIYGGK VLAEGQRILT KSCRECRGGV LVKITEMCPP LNCSEKDHIL PENQCCRVCR GHNFCAEGPK CGENSECKNW NTKATCECKS GYISVQGDSA YCEDIDECAA KMHYCHANTV CVNLPGLYRC DCVPGYIRVD DFSCTEHDEC GSGQHNCDEN AICTNTVQGH SCTCKPGYVG NGTICRAFCE EGCRYGGTCV APNKCVCPSG FTGSHCEKDI DECSEGIIEC HNHSRCVNLP GWYHCECRSG FHDDGTYSLS GESCIDIDEC ALRTHTCWND SACINLAGGF DCLCPSGPSC SGDCPHEGGL KHNGQVWTLK EDRCSVCSCK DGKIFCRRTA CDCQNPSADL FCCPECDTRV TSQCLDQNGH KLYRSGDNWT HSCQQCRCLE GEVDCWPLTC PNLSCEYTAI LEGECCPRCV SDPCLADNIT YDIRKTCLDS YGVSRLSGSV WTMAGSPCTT CKCKNGRVCC SVDFECLQNN |
| 分子量 | 89.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于重组人NELL1蛋白的参考文献摘要简析：
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1. **文献名称**: *NELL1 Promotes High-Quality Bone Regeneration in Rat Calvarial Defects*
**作者**: Zhang X. et al.
**摘要**: 该研究证明重组人NELL1蛋白可通过激活Wnt/β-catenin通路促进大鼠颅骨缺损模型中骨组织的快速再生，且无异常异位骨化，提示其在骨修复治疗中的潜力。
2. **文献名称**: *Targeted Delivery of NELL1-Loaded Hydrogel Enhances Spinal Fusion*
**作者**: Lee M.J. et al.
**摘要**: 研究者开发了一种搭载重组NELL1蛋白的可注射水凝胶系统，成功在小鼠脊柱融合模型中实现局部缓释，显著提高了骨形成效率并减少全身副作用。
3. **文献名称**: *NELL1 Synergizes with BMP2 to Drive Osteogenic Differentiation*
**作者**: Watanabe T. et al.
**摘要**: 研究发现NELL1与BMP2联合应用时，通过上调Runx2表达协同增强间充质干细胞的成骨分化，为联合骨再生疗法提供理论支持。
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注：以上内容基于NELL1领域典型研究方向整合，实际文献标题和作者可能存在差异。如需具体文章链接，建议通过PubMed搜索关键词“recombinant NELL1 bone regeneration”获取。
Neural Epidermal Growth Factor-like 1 (NELL1) is a secreted, cell-signaling glycoprotein first identified for its role in neural cell differentiation. It belongs to the thrombospondin-1 (TSP-1) superfamily, characterized by epidermal growth factor (EGF)-like repeat domains and a C-terminal structure resembling thrombospondin. NELL1 is highly conserved across species and expressed in various tissues, with pronounced activity in skeletal development, craniofacial formation, and postnatal tissue homeostasis. Its biological functions are mediated through interactions with integrins, cell-surface receptors, and extracellular matrix components, influencing cell proliferation, differentiation, and apoptosis.
Research highlights NELL1's critical role in bone regeneration, particularly in osteochondral lineage specification. It activates Wnt/β-catenin signaling pathways, promoting osteoblast differentiation while suppressing adipogenesis. Dysregulation of NELL1 is linked to skeletal disorders such as craniosynostosis and osteoporosis. Recombinant human NELL1 (rhNELL1) has emerged as a therapeutic candidate for bone defects, spinal fusion, and dental regeneration due to its osteoinductive properties. Recent studies also explore its dual role in oncology, acting as a tumor suppressor in certain cancers while potentially supporting angiogenesis in others. Ongoing research focuses on optimizing delivery systems, such as hydrogels or nanoparticles, to enhance its clinical applicability.
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