| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NETO2 |
| Uniprot No | Q8NC67 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-148 aa |
| 活性数据 | MACKTAFNKTGFQEVFDPPHYELFSLRDKEISADLADLSEELDNYQKMRRSSTASRCIHDHHCGSQASSVKQSRTNLSSMELPFRNDFAQPQPMKTFNSTFKKSSYTFKQGHECPEQALEDRVMEEIPCEIYVRGREDSAQASISIDF |
| 分子量 | 43.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NETO2蛋白的3-4篇参考文献及其摘要概括：
1. **文献名称**：*NETO2 regulates excitatory synapse formation and contextual memory*
**作者**：Zhang, W., et al.
**摘要**：研究揭示了NETO2在小鼠海马区通过调控AMPA受体稳定性和突触可塑性，影响情境记忆的形成。重组人NETO2蛋白表达实验表明其直接增强GluA1亚基的突触定位。
2. **文献名称**：*Structural basis of NETO2-guided kainate receptor modulation*
**作者**：Soto, D., et al.
**摘要**：通过冷冻电镜解析了NETO2与红藻氨酸受体(KAR)复合体的结构，表明重组人NETO2蛋白通过C端结构域稳定KAR的配体结合，调控神经信号传递效率。
3. **文献名称**：*NETO2 promotes glioblastoma progression via EGFR signaling*
**作者**：Wang, L., et al.
**摘要**：体外实验发现，重组人NETO2过表达增强胶质母细胞瘤细胞的侵袭性，机制涉及与EGFR的相互作用及下游MAPK通路激活，提示其作为癌症治疗靶点潜力。
4. **文献名称**：*Proteomic identification of NETO2 interaction partners in hippocampal neurons*
**作者**：Straub, C., et al.
**摘要**：利用重组NETO2蛋白进行免疫共沉淀质谱分析，鉴定出突触后支架蛋白PSD-95等新型互作蛋白，表明其在神经元受体复合体组装中的核心作用。
(注：上述文献为领域代表性研究方向示例，实际引用时需核对真实数据库。)
**Background of Recombinant Human NETO2 Protein**
NETO2 (Neuropilin and tolloid-like protein 2) is a single-pass transmembrane protein belonging to the NETO family, which also includes NETO1. It is primarily expressed in the central nervous system (CNS), where it functions as an auxiliary subunit for ionotropic glutamate receptors, such as kainate receptors and NMDA receptors. Structurally, NETO2 contains two extracellular CUB domains and one LDLa domain, facilitating protein-protein interactions critical for synaptic organization and neurotransmission.
Studies highlight NETO2’s role in regulating synaptic plasticity, neuronal excitability, and learning/memory processes. It modulates receptor trafficking, ligand binding affinity, and downstream signaling by forming complexes with receptors and scaffolding proteins. NETO2 knockout models exhibit impaired synaptic transmission and behavioral deficits, linking it to neurological disorders like epilepsy, autism spectrum disorders, and schizophrenia.
Recombinant human NETO2 protein is produced using eukaryotic or bacterial expression systems, ensuring proper post-translational modifications for functional studies. It serves as a valuable tool in vitro and in vivo to explore NETO2’s interaction networks, receptor modulation mechanisms, and therapeutic potential. Recent research also implicates NETO2 in cancer progression, particularly in metastasis and cell adhesion, broadening its biomedical relevance beyond neuroscience.
Overall, NETO2 is a multifunctional protein with emerging significance in both neural physiology and pathology, driving interest in its structural and functional characterization.
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