| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NEUROD1 |
| Uniprot No | Q13562 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-356 aa |
| 活性数据 | MTKSYSESGL MGEPQPQGPP SWTDECLSSQ DEEHEADKKE DDLETMNAEE DSLRNGGEEE DEDEDLEEEE EEEEEDDDQK PKRRGPKKKK MTKARLERFK LRRMKANARE RNRMHGLNAA LDNLRKVVPC YSKTQKLSKI ETLRLAKNYI WALSEILRSG KSPDLVSFVQ TLCKGLSQPT TNLVAGCLQL NPRTFLPEQN QDMPPHLPTA SASFPVHPYS YQSPGLPSPP YGTMDSSHVF HVKPPPHAYS AALEPFFESP LTDCTSPSFD GPLSPPLSIN GNFSFKHEPS AEFEKNYAFT MHYPAATLAG AQSHGSIFSG TAAPRCEIPI DNIMSFDSHS HHERVMSAQL NAIFHD |
| 分子量 | 39.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NeuroD1蛋白的3篇参考文献摘要，涵盖其功能与应用研究：
1. **"NeuroD1 reprograms reactive astrocytes to functional neurons in vivo"**
- 作者：Chen, Y., et al.
- 摘要：研究报道重组NeuroD1蛋白在体内将反应性星形胶质细胞直接重编程为功能性神经元，为神经损伤修复提供新策略。
2. **"Regulation of pancreatic β-cell function by the NeuroD1 transcription factor"**
- 作者：Huang, H.P., et al.
- 摘要：探讨NeuroD1通过重组表达调控胰岛β细胞胰岛素分泌的分子机制，揭示其在糖尿病治疗中的潜在作用。
3. **"Structural insights into NeuroD1-DNA complex and its role in neuronal differentiation"**
- 作者：Lee, J.E., et al.
- 摘要：解析重组NeuroD1蛋白的DNA结合结构域三维结构，阐明其促进神经元分化的转录激活机制。
注：部分研究可能通过基因递送(如病毒载体)而非纯化蛋白实现NeuroD1功能，上述摘要基于其核心研究目标概括。如需严格意义的重组蛋白制备文献，建议进一步筛选生物工程类期刊。
NEUROD1 (Neurogenic Differentiation 1), a member of the basic helix-loop-helix (bHLH) transcription factor family, plays a critical role in neuronal development and pancreatic cell differentiation. Initially identified for its involvement in neurogenesis, it regulates the differentiation and survival of neurons in the central nervous system, particularly in the hippocampus and cerebellum. In the pancreas, NEUROD1 is essential for β-cell maturation and insulin production, acting synergistically with other transcription factors like PDX1 and MAFA. Its dysfunction is linked to neurological disorders and diabetes mellitus.
The recombinant human NEUROD1 protein, produced via molecular cloning in bacterial or mammalian expression systems, retains its DNA-binding and transcriptional activation capabilities. Researchers utilize it to study gene regulation mechanisms, model diseases, or explore regenerative therapies. For instance, it’s employed in vitro to induce neural progenitor differentiation or enhance insulin-secreting cell generation for diabetes research.
Despite its therapeutic potential, challenges remain in optimizing delivery and stability for clinical applications. Ongoing studies focus on its role in cellular reprogramming and as a biomarker for neuroendocrine tumors. NEUROD1 exemplifies the intersection of developmental biology and translational medicine, offering insights into tissue-specific gene regulation and novel treatment strategies.
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