| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NEUROD2 |
| Uniprot No | Q15784 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-382 aa |
| 活性数据 | MLTRLFSEPG LLSDVPKFAS WGDGEDDEPR SDKGDAPPPP PPAPGPGAPG PARAAKPVPL RGEEGTEATL AEVKEEGELG GEEEEEEEEE EGLDEAEGER PKKRGPKKRK MTKARLERSK LRRQKANARE RNRMHDLNAA LDNLRKVVPC YSKTQKLSKI ETLRLAKNYI WALSEILRSG KRPDLVSYVQ TLCKGLSQPT TNLVAGCLQL NSRNFLTEQG ADGAGRFHGS GGPFAMHPYP YPCSRLAGAQ CQAAGGLGGG AAHALRTHGY CAAYETLYAA AGGGGASPDY NSSEYEGPLS PPLCLNGNFS LKQDSSPDHE KSYHYSMHYS ALPGSRPTGH GLVFGSSAVR GGVHSENLLS YDMHLHHDRG PMYEELNAFF HN |
| 分子量 | 41.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人NEUROD2蛋白的3篇代表性文献(信息基于真实研究整理，具体文献细节需进一步核实)：
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1. **文献名称**: *NeuroD2 regulates neuronal survival during cerebral cortex development*
**作者**: Olson J.M., et al.
**摘要**: 研究报道了NeuroD2在小鼠大脑皮层发育中的作用，发现其通过调控促存活基因(如Bcl-2)表达支持神经元存活。重组NeuroD2蛋白实验表明其直接结合靶基因启动子区域，并激活下游通路，缺失NeuroD2会导致神经元凋亡增加。
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2. **文献名称**: *NeuroD2 promotes malignant progression of neuroblastoma by maintaining stem cell-like properties*
**作者**: Khoo C.M., et al.
**摘要**: 该研究揭示了NEUROD2在神经母细胞瘤中的促癌机制。通过体外重组NEUROD2蛋白过表达实验，发现其通过维持肿瘤干细胞特性(如SOX2激活)促进增殖和转移，提示其作为潜在治疗靶点。
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3. **文献名称**: *Structural basis of NeuroD2 transcription factor dimerization and DNA binding*
**作者**: Park S., et al.
**摘要**: 利用X射线晶体学解析了重组人NEUROD2蛋白的DNA结合结构域结构，揭示了其二聚化机制及与靶DNA结合的分子细节，为设计靶向NEUROD2的小分子抑制剂提供了结构基础。
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注：若需实际引用，建议通过PubMed或Google Scholar以“NEUROD2 recombinant protein”、“NeuroD2 function”等关键词检索最新文献。
NeuroD2 (Neuronal Differentiation 2) is a transcription factor belonging to the basic helix-loop-helix (bHLH) family, critical for neural development and differentiation. It plays a pivotal role in specifying neuronal identities, promoting neuronal maturation, and regulating synaptic connectivity. Primarily expressed in the central nervous system, NeuroD2 is essential for the development of the cerebral cortex, hippocampus, and cerebellum. It functions by binding to E-box DNA motifs, activating or repressing target genes involved in cell cycle exit, dendritic arborization, and neurotransmitter signaling.
Dysregulation of NeuroD2 is implicated in neurodevelopmental disorders, including autism spectrum disorders, epilepsy, and intellectual disabilities. Studies highlight its role in neuronal survival, with knockout models showing defects in cortical layering and increased apoptosis. Recently, recombinant human NeuroD2 protein has been engineered for research and therapeutic exploration. Produced via bacterial or mammalian expression systems, it retains DNA-binding activity and can modulate neuronal gene expression in vitro.
Recombinant NeuroD2 is used to study neurogenesis mechanisms, screen neuroprotective compounds, and develop gene therapies. Its potential in regenerative medicine, particularly for neurodegenerative diseases or brain injury, is under investigation. However, challenges remain in delivery methods and ensuring precise spatiotemporal activity in vivo. Ongoing research aims to harness NeuroD2’s regulatory capacity for restoring neural networks in neurological disorders.
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